<p>Zileuton (ZLN) is a pharmaceutical agent utilized to manage inflammation-related disorders, including chronic obstructive pulmonary disease, upper respiratory tract conditions, and various dermatoses. It functions by inhibiting the synthesis of leukotrienes, which are mediators that contribute to edema, inflammation, mucus production, and bronchoconstriction. An overdose of ZLN can lead to significant adverse effects in patients; therefore, precise measurement of ZLN concentrations is essential. In the current study, copper-doped tungsten oxide (Cu-WO₃) nano-materials were employed to design carbon paste and prepare electrode materials for ZLN detection. The synthesized Cu-WO₃ nanomaterials were characterized using different analytical techniques. Cyclic and square wave voltammetry were performed to check the electrochemical behavior of ZLN on bare as well as modified electrodes. Results displayed a great enhancement in the peak current of ZLN, showing effect on sensitivity, specificity, and reliability for ZLN detection. Thus, the Cu-WO₃-modified electrode demonstrated much faster electron transport kinetics for the catalytic oxidation process than the unmodified carbon paste electrode. The studies on kinetics of oxidation under optimized conditions, the modified electrode exhibited a notable linear detection range of 5.0 × 10^–8 to 8.0 × 10^–6&#xa0;M, with a limit of detection of 7.5&#xa0;nM and a limit of quantification of 25.1&#xa0;nM. For the electrochemical oxidation of ZLN, parameters such as the heterogeneous rate constant, electron transfer coefficient, and number of electrons involved were determined. The developed sensor was also employed to analyze ZLN in real sample matrices, yielding satisfactory results.</p> Graphical Abstract <p></p>