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Introduction <p>Fanconi anemia supplementation group I (FANCI), one of the Fanconi family proteins, may be closely related to the malignant progression of glioma cells. Here, we sought to validate the expression of FANCI in glioma cells and its role in regulating the Akt signaling pathway in the malignant progression of glioma cells.</p> Methods <p>The expression of FANCI in glioma cells was analyzed by bioinformatics and microarray. Lentivirus transfection was used to regulate the expression of FANCI in glioma cells. CCK-8, EdU, Transwell, and flow cytometry were used to observe the effect of FANCI on the malignant progression of glioma cells.</p> Results <p>We found that low expression of FANCI inhibited the proliferation, migration and invasion of human glioma cells, and promoted cell apoptosis. However, overexpression of FANCI produced the opposite effect. We also found that low expression of FANCI inhibited the expression of P-Akt and B-cell lymphoma-2 (Bcl-2). Finally, we validated these in vitro results in a xenograft mouse model.</p> Conclusion <p>FANCI can inhibit the development of glioma by inhibiting Akt/Bcl-2 signaling pathway.</p>

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FANCI is involved in the malignant progression of glioma cells by regulating the Akt/Bcl-2 signaling pathway

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月13日

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  • Hanyun Li,
  • Yinrui Liu,
  • Mingyao Wang,
  • Shaoshao Xu,
  • Jilan Liu,
  • Xianyun Qin,
  • Banban Li,
  • Jikui Sun,
  • Yan Liu,
  • Feng Jin

摘要

Introduction

Fanconi anemia supplementation group I (FANCI), one of the Fanconi family proteins, may be closely related to the malignant progression of glioma cells. Here, we sought to validate the expression of FANCI in glioma cells and its role in regulating the Akt signaling pathway in the malignant progression of glioma cells.

Methods

The expression of FANCI in glioma cells was analyzed by bioinformatics and microarray. Lentivirus transfection was used to regulate the expression of FANCI in glioma cells. CCK-8, EdU, Transwell, and flow cytometry were used to observe the effect of FANCI on the malignant progression of glioma cells.

Results

We found that low expression of FANCI inhibited the proliferation, migration and invasion of human glioma cells, and promoted cell apoptosis. However, overexpression of FANCI produced the opposite effect. We also found that low expression of FANCI inhibited the expression of P-Akt and B-cell lymphoma-2 (Bcl-2). Finally, we validated these in vitro results in a xenograft mouse model.

Conclusion

FANCI can inhibit the development of glioma by inhibiting Akt/Bcl-2 signaling pathway.