Scholarlink

<p>This study aims to elucidate the functional mechanisms of anionic cellulase nanoparticles, a sustainable nanomaterial with diverse physiochemical and biomedical applications. The versatile functionality of these biopolymers is primarily dictated by their surface charge characteristics. Preliminary spectral analysis in the ultraviolet (UV) range, and Fourier-transform infrared (FT-IR) spectroscopy was used to confirm characteristic functional groups. Zeta potential (ζ-potential) and size distribution were measured via Zetasizer to verify particle scale and surface charge. Ultrastructural analysis was performed using scanning and transmission electron microscopy (SEM and TEM). Biomedical efficacy was evaluated through antioxidant, biocompatibility testing on human erythrocytes and lymphocytes, antimicrobial assays and anticancer against Caco-2 colorectal cancer cells. Zetasizer analysis verified that the cellulase particles are nanometer-scale and possess a negative surface charge, while SEM and TEM manifested that the NPs aggregate into a roughly globular shape. The nanoparticles exhibited an enhanced antioxidant capacity alongside negligible cytotoxicity toward human erythrocytes and lymphocyte proliferation. At a concentration of 500&#xa0;µg/mL, the biopolymer demonstrated statistically significant (<i>p</i> &lt; 0.05) antimicrobial efficacy against the tested strains of Gram-positive bacteria and fungi. Furthermore, comet assay analysis revealed that the administration of the negatively charged cellulase induced apoptosis in Caco-2 colorectal cancer cells. This cytotoxic response is attributed to electrostatic attractions and interfacial interactions between charges. Collectively, these findings emphasize anionic cellulase as a promising nanotherapeutic agent for biomedical applications, maybe offering a safe avenue for upregulating the immune response. Their therapeutic capacity is intimately linked to their ζ-potential characteristics.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Anionic Cellulase Enzyme Nanoparticles as an Antioxidant, Antimicrobial, and Antitumor Agent (Caco-2 Colon Cancer Cells): Role of Zeta Potential

  • 期刊论文

  • 发表日期：

    2026年7月16日

查看全文
  • Sara Mohamed Mahmoud,
  • Hager Abd El-Hakim Nawar,
  • Mohamed Abd El-Hady Omar,
  • Safwat Hassan Ali

摘要

This study aims to elucidate the functional mechanisms of anionic cellulase nanoparticles, a sustainable nanomaterial with diverse physiochemical and biomedical applications. The versatile functionality of these biopolymers is primarily dictated by their surface charge characteristics. Preliminary spectral analysis in the ultraviolet (UV) range, and Fourier-transform infrared (FT-IR) spectroscopy was used to confirm characteristic functional groups. Zeta potential (ζ-potential) and size distribution were measured via Zetasizer to verify particle scale and surface charge. Ultrastructural analysis was performed using scanning and transmission electron microscopy (SEM and TEM). Biomedical efficacy was evaluated through antioxidant, biocompatibility testing on human erythrocytes and lymphocytes, antimicrobial assays and anticancer against Caco-2 colorectal cancer cells. Zetasizer analysis verified that the cellulase particles are nanometer-scale and possess a negative surface charge, while SEM and TEM manifested that the NPs aggregate into a roughly globular shape. The nanoparticles exhibited an enhanced antioxidant capacity alongside negligible cytotoxicity toward human erythrocytes and lymphocyte proliferation. At a concentration of 500 µg/mL, the biopolymer demonstrated statistically significant (p < 0.05) antimicrobial efficacy against the tested strains of Gram-positive bacteria and fungi. Furthermore, comet assay analysis revealed that the administration of the negatively charged cellulase induced apoptosis in Caco-2 colorectal cancer cells. This cytotoxic response is attributed to electrostatic attractions and interfacial interactions between charges. Collectively, these findings emphasize anionic cellulase as a promising nanotherapeutic agent for biomedical applications, maybe offering a safe avenue for upregulating the immune response. Their therapeutic capacity is intimately linked to their ζ-potential characteristics.

Graphical Abstract