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<p>Sugarcane (<i>Saccharum officinarum</i>) is a commercial agricultural crop utilized for food and bioenergy production worldwide. However, fungal pathogens significantly impact its yield and quality. Current management strategies often lack sustainability and efficacy. Silver nanoparticles are potent agents for antifungal activity by disrupting the cell membrane of fungal pathogens more effectively. In this study, silver nanoparticles were synthesized using the leaves of <i>Ocimum sanctum.</i> Synthesized <i>O.sanctum-mediated</i>nanoparticles were mixed with neem oil to formulate a neem oil-basedgreen nanoemulsion (NONE) by using the sonochemical method. The fungal strains isolated from infected sugarcane leaves were identified by molecular methods. Green nanoemulsionwas characterizedby using Zeta potential, DLS, FTIR, UV-Visible spectroscopy, XRD, FESEM, and EDAX analysis. UV–visible spectroscopy confirmed the synthesis of silver nanoparticles with a characteristic absorbance at 400–500&#xa0;nm, while XRD analysis revealed a crystalline nature. FESEM analysis showed uniformly distributed nanosized particles within the neem oil-based nanoemulsion. Zeta potential results showed negative charge of -40.5 mV and DLS showed particle size as 250.1&#xa0;nm. NONE has effectively suppressed the growth of fungi, namely <i>Rhizopus arrhizus</i>, <i>Rhizopus microsporus</i>, <i>Rhizopus delemar</i>,<i> and Curvulariaeragrostidis</i> isolated from infected sugarcane leaves. The nanoemulsion exhibited strong dose-dependent antifungal activity in <i>Rhizopus arrhizus</i>, <i>R. microsporus</i>, <i>R. delemar</i>, and <i>Curvularia eragrostidis</i>, achieving maximum growth inhibition of 91.81%, 94.18%, 90.33%, and 91.03%, respectively, at a concentration of 100 µL/mL.The results suggest that NONEis a promising alternative to commercial antifungal agents and can promote sustainable agriculture at low cost and without causing any side effects to flora and fauna present in the ecosystem.</p>

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Neem Oil Based Nanoemulsion as a Delivery System for Ocimum sanctum Mediated Nanoparticles to Control Sugarcane Pathogens

  • 期刊论文

  • 发表日期：

    2026年6月30日

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  • V. Divyashree,
  • S. Ranjani,
  • S. Hemalatha

摘要

Sugarcane (Saccharum officinarum) is a commercial agricultural crop utilized for food and bioenergy production worldwide. However, fungal pathogens significantly impact its yield and quality. Current management strategies often lack sustainability and efficacy. Silver nanoparticles are potent agents for antifungal activity by disrupting the cell membrane of fungal pathogens more effectively. In this study, silver nanoparticles were synthesized using the leaves of Ocimum sanctum. Synthesized O.sanctum-mediatednanoparticles were mixed with neem oil to formulate a neem oil-basedgreen nanoemulsion (NONE) by using the sonochemical method. The fungal strains isolated from infected sugarcane leaves were identified by molecular methods. Green nanoemulsionwas characterizedby using Zeta potential, DLS, FTIR, UV-Visible spectroscopy, XRD, FESEM, and EDAX analysis. UV–visible spectroscopy confirmed the synthesis of silver nanoparticles with a characteristic absorbance at 400–500 nm, while XRD analysis revealed a crystalline nature. FESEM analysis showed uniformly distributed nanosized particles within the neem oil-based nanoemulsion. Zeta potential results showed negative charge of -40.5 mV and DLS showed particle size as 250.1 nm. NONE has effectively suppressed the growth of fungi, namely Rhizopus arrhizus, Rhizopus microsporus, Rhizopus delemar, and Curvulariaeragrostidis isolated from infected sugarcane leaves. The nanoemulsion exhibited strong dose-dependent antifungal activity in Rhizopus arrhizus, R. microsporus, R. delemar, and Curvularia eragrostidis, achieving maximum growth inhibition of 91.81%, 94.18%, 90.33%, and 91.03%, respectively, at a concentration of 100 µL/mL.The results suggest that NONEis a promising alternative to commercial antifungal agents and can promote sustainable agriculture at low cost and without causing any side effects to flora and fauna present in the ecosystem.