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<p>This study developed a simple green approach for producing bimetallic nanoparticles of two metals the silver and gold (Ag/Au BNPs) via methanolic extract of <i>Heliotropium eichwaldi</i> L (HE). The metals reduction to stable BNPs after 24&#xa0;h of incubation at 40&#xa0;°C under optimum conditions of pH 5, 1 mM salts concentration, and 1 mL HE extract, confirm the green synthesis of Ag/Au BNPs. Different characterizations i.e. UV visible spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and energy dispersive X-ray (EDX) were used to confirm the synthesis, size, shape, and elemental composition. In the current research work stable polydisperse, irregular rod-shaped, crystal type NPs with a size of 6&#xa0;nm and a max UV absorbance of 532&#xa0;nm were reported. According to EDX, the Ag signal 4.20% and Au signal 26.65% were reported. According to the comet assay employing blood cells, BNPs induced significantly more DNA damage (217.31) than the plant extract. The total comet score (TCS) was used to calculate the comet assay. Dose-dependent cytotoxicity was seen in the MTT utilizing baby hamster kidney-21 cells (BHK-21) assay, with doxorubicin, revealing 15.8 ± 0.7% cell viability and BNPs 23 ± 2.3% and proved to be a more significant cytotoxic agent against BHK-21 cells than plant extract. Doxorubicin, Ag/Au BNPs, and HE extract concentrations that reduce 50% activirty of BHK-21 cells were 134 ± 0.14, 296 ± 0.21, and 527 ± 0.23&#xa0;µg/ml, respectively. Overall, our findings highlighted the BNPs with promise latent as cytotoxic properties, and the green technique allows us to recommend these BNPs as good agents for the creation of pharmaceuticals useful in cancer treatment.</p>

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Exploring the Anticancer Potential of Green Silver-gold Allied Bimetallic Nanoparticles on BHK-21 Cancer Cells Via Genotoxic Approach

  • 期刊论文

  • 发表日期：

    2026年7月4日

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  • Mushtaq Ahmed,
  • Naila Sher,
  • Nadia Mushtaq

摘要

This study developed a simple green approach for producing bimetallic nanoparticles of two metals the silver and gold (Ag/Au BNPs) via methanolic extract of Heliotropium eichwaldi L (HE). The metals reduction to stable BNPs after 24 h of incubation at 40 °C under optimum conditions of pH 5, 1 mM salts concentration, and 1 mL HE extract, confirm the green synthesis of Ag/Au BNPs. Different characterizations i.e. UV visible spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and energy dispersive X-ray (EDX) were used to confirm the synthesis, size, shape, and elemental composition. In the current research work stable polydisperse, irregular rod-shaped, crystal type NPs with a size of 6 nm and a max UV absorbance of 532 nm were reported. According to EDX, the Ag signal 4.20% and Au signal 26.65% were reported. According to the comet assay employing blood cells, BNPs induced significantly more DNA damage (217.31) than the plant extract. The total comet score (TCS) was used to calculate the comet assay. Dose-dependent cytotoxicity was seen in the MTT utilizing baby hamster kidney-21 cells (BHK-21) assay, with doxorubicin, revealing 15.8 ± 0.7% cell viability and BNPs 23 ± 2.3% and proved to be a more significant cytotoxic agent against BHK-21 cells than plant extract. Doxorubicin, Ag/Au BNPs, and HE extract concentrations that reduce 50% activirty of BHK-21 cells were 134 ± 0.14, 296 ± 0.21, and 527 ± 0.23 µg/ml, respectively. Overall, our findings highlighted the BNPs with promise latent as cytotoxic properties, and the green technique allows us to recommend these BNPs as good agents for the creation of pharmaceuticals useful in cancer treatment.