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<p>The increasing prevalence of multidrug-resistant bacterial infections necessitates alternative antibacterial strategies with improved efficacy and controllability. In this study, we introduce a Self-Bacterial Panning (SBP) methodology, guided by a Fight Bacteria with Bacteria (F2B) framework, to enrich bacterial surface-associated proteins from <i>Klebsiella pneumoniae</i> (KP) for nanoparticle functionalization. The SBP process involves iterative incubation of soluble bacterial protein extracts with intact KP cells, followed by recovery and re-enrichment of protein fractions that remain associated with the bacterial surface. These SBP-enriched proteins were subsequently utilized as both reducing and capping agents for the synthesis of protein-mediated silver nanoparticles (AgNPs). Compared with nanoparticles synthesized using non-enriched bacterial proteins (Reg-KP-AgNPs) or citrate capping agents, SBP-derived AgNPs (KP-SBP2-AgNPs) demonstrated consistently enhanced antibacterial activity against KP, as evidenced by larger zones of inhibition and lower minimum inhibitory concentrations. Increased antibacterial effects were also observed against additional bacterial species, including <i>Staphylococcus aureus</i>, <i>Burkholderia cenocepacia</i>, and <i>Pseudomonas aeruginosa</i>, although not uniformly across all tested strains. These findings suggest that SBP-derived protein coatings enhance nanoparticle–bacteria interactions rather than confer strict bacterial specificity. Overall, this study demonstrates that SBP provides a simple, time- and cost-efficient strategy for generating protein-functionalized silver nanoparticles with improved antibacterial performance. This approach offers a practical and adaptable platform for enhancing nanoparticle–bacteria interactions and advancing the development of new antibacterial nanomaterials.</p>

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Enhancing the Antibacterial Activity of Silver Nanoparticles Using Self-Bacterial Panning

  • 期刊论文

  • 发表日期：

    2026年5月20日

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  • James Lee Cho,
  • William Joseph Dodson,
  • Lauren Hanna Sloneker,
  • Yacoub Qamar,
  • William Yang,
  • Fajr Fayed,
  • Samyra Dominique Gipson-Gordones

摘要

The increasing prevalence of multidrug-resistant bacterial infections necessitates alternative antibacterial strategies with improved efficacy and controllability. In this study, we introduce a Self-Bacterial Panning (SBP) methodology, guided by a Fight Bacteria with Bacteria (F2B) framework, to enrich bacterial surface-associated proteins from Klebsiella pneumoniae (KP) for nanoparticle functionalization. The SBP process involves iterative incubation of soluble bacterial protein extracts with intact KP cells, followed by recovery and re-enrichment of protein fractions that remain associated with the bacterial surface. These SBP-enriched proteins were subsequently utilized as both reducing and capping agents for the synthesis of protein-mediated silver nanoparticles (AgNPs). Compared with nanoparticles synthesized using non-enriched bacterial proteins (Reg-KP-AgNPs) or citrate capping agents, SBP-derived AgNPs (KP-SBP2-AgNPs) demonstrated consistently enhanced antibacterial activity against KP, as evidenced by larger zones of inhibition and lower minimum inhibitory concentrations. Increased antibacterial effects were also observed against additional bacterial species, including Staphylococcus aureus, Burkholderia cenocepacia, and Pseudomonas aeruginosa, although not uniformly across all tested strains. These findings suggest that SBP-derived protein coatings enhance nanoparticle–bacteria interactions rather than confer strict bacterial specificity. Overall, this study demonstrates that SBP provides a simple, time- and cost-efficient strategy for generating protein-functionalized silver nanoparticles with improved antibacterial performance. This approach offers a practical and adaptable platform for enhancing nanoparticle–bacteria interactions and advancing the development of new antibacterial nanomaterials.