Scholarlink

<p>The green synthesis of ZnO nanoparticles (ZnONPs) has gained significant interest due to their antimicrobial activity and potential as a fertilizer for enhancing plant growth. In this study, ZnONPs were synthesized using diazotrophic <i>Bacillus subtilis</i> extract with ZnSO₄·7&#xa0;H₂O as a precursor and characterized using UV-spectrophotometry, FTIR, and transmission electron microscopy (TEM). The synthesized ZnONPs showed maximum absorbance at 375&#xa0;nm and exhibited hexagonal rod-shaped morphology with sizes ranging from 50 to 150&#xa0;nm in length. FTIR analysis confirmed the reduction of zinc ions by bacterial metabolites. The biosynthesized ZnONPs demonstrated broad-spectrum antimicrobial activity against phytopathogenic bacteria and fungi, with minimum inhibitory concentrations (MIC) ranging from 10 to 156&#xa0;µg/mL. <i>Pseudomonas aeruginosa</i> was the most susceptible (MIC: 10&#xa0;µg/mL), while <i>B. cereus</i> and <i>(A) flavus</i> were more resistant (MIC: 156&#xa0;µg/mL). The effects of ZnONPs on seed germination and seedling growth were concentration- and species-dependent. Optimal concentrations differed between crops: wheat showed enhanced germination indices and biomass at 5–15&#xa0;mg/L with maximum benefits at 10&#xa0;mg/L, while faba bean responded optimally only at 5&#xa0;mg/L. Higher concentrations (20&#xa0;mg/L) inhibited germination and seedling growth in both species. These findings demonstrate that ZnONPs synthesized by diazotrophic <i>(B) subtilis</i> offer a promising bio-inspired strategy for controlling phytopathogenic microbes while promoting plant growth at appropriate concentrations.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biological Activity of Zinc Oxide Nanoparticles Synthesized by Zinc Tolerant Diazotrophic Bacillus subtilis

  • 期刊论文

  • 发表日期：

    2026年5月13日

查看全文
  • Ahmed Salah,
  • Abd El-Latif Hesham,
  • Naemia M. Yousef,
  • Asmaa M. M. Mawad

摘要

The green synthesis of ZnO nanoparticles (ZnONPs) has gained significant interest due to their antimicrobial activity and potential as a fertilizer for enhancing plant growth. In this study, ZnONPs were synthesized using diazotrophic Bacillus subtilis extract with ZnSO₄·7 H₂O as a precursor and characterized using UV-spectrophotometry, FTIR, and transmission electron microscopy (TEM). The synthesized ZnONPs showed maximum absorbance at 375 nm and exhibited hexagonal rod-shaped morphology with sizes ranging from 50 to 150 nm in length. FTIR analysis confirmed the reduction of zinc ions by bacterial metabolites. The biosynthesized ZnONPs demonstrated broad-spectrum antimicrobial activity against phytopathogenic bacteria and fungi, with minimum inhibitory concentrations (MIC) ranging from 10 to 156 µg/mL. Pseudomonas aeruginosa was the most susceptible (MIC: 10 µg/mL), while B. cereus and (A) flavus were more resistant (MIC: 156 µg/mL). The effects of ZnONPs on seed germination and seedling growth were concentration- and species-dependent. Optimal concentrations differed between crops: wheat showed enhanced germination indices and biomass at 5–15 mg/L with maximum benefits at 10 mg/L, while faba bean responded optimally only at 5 mg/L. Higher concentrations (20 mg/L) inhibited germination and seedling growth in both species. These findings demonstrate that ZnONPs synthesized by diazotrophic (B) subtilis offer a promising bio-inspired strategy for controlling phytopathogenic microbes while promoting plant growth at appropriate concentrations.

Graphical Abstract