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<p>Recently, biocompatible nanoimaging platforms are of increasing interest for cancer field. However, most of systems rely on synthetic materials, which often show the limited in vivo stability and rapid clearance by immune system. Recently many nanoplatforms choose bio-membrane camouflaged methods to enhance their immune escape ability in vivo. Here, we report a biocompatible nano platform using erythrocyte incorporating near-infrared (NIR) quantum dots (QDs) for cancer. The particles were prepared under 100&#xa0;nm to fit the enhanced permeability and retention (EPR) effect of cancer cells. All erythrocyte nano particles were prepared from bare erythrocyte membrane without hemoglobin using the hypotonic swelling method. All nanoparticles purified size exclusion chromatography after preparation and each fraction were used for the identification. Stable entrapment of QDs within EDNs was validated by simultaneous detection of erythrocyte membrane protein (595&#xa0;nm, Bradford reagent) and characteristic NIR QD absorbance (980&#xa0;nm) within identical chromatographic fractions. The prepared nanoplatform was characterized with DLS analysis. Furthermore, a pilot in vivo imaging study using a tumor-bearing mouse demonstrated the feasibility of EDNs-QDs for NIR bioimaging. Taken together, these results demonstrate that erythrocyte membrane derived nanoparticles can be suggested as a biocompatible and spectrally validated nanoimaging platform, supporting their potential utility in bioimaging applications.</p>

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Physicochemical and Spectral Validation of Erythrocyte Membrane-Derived Nanoimaging Platforms for NIR Bioimaging

  • 期刊论文

  • 发表日期：

    2026年3月31日

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  • Moon Jung Choi,
  • Yong Serk Park

摘要

Recently, biocompatible nanoimaging platforms are of increasing interest for cancer field. However, most of systems rely on synthetic materials, which often show the limited in vivo stability and rapid clearance by immune system. Recently many nanoplatforms choose bio-membrane camouflaged methods to enhance their immune escape ability in vivo. Here, we report a biocompatible nano platform using erythrocyte incorporating near-infrared (NIR) quantum dots (QDs) for cancer. The particles were prepared under 100 nm to fit the enhanced permeability and retention (EPR) effect of cancer cells. All erythrocyte nano particles were prepared from bare erythrocyte membrane without hemoglobin using the hypotonic swelling method. All nanoparticles purified size exclusion chromatography after preparation and each fraction were used for the identification. Stable entrapment of QDs within EDNs was validated by simultaneous detection of erythrocyte membrane protein (595 nm, Bradford reagent) and characteristic NIR QD absorbance (980 nm) within identical chromatographic fractions. The prepared nanoplatform was characterized with DLS analysis. Furthermore, a pilot in vivo imaging study using a tumor-bearing mouse demonstrated the feasibility of EDNs-QDs for NIR bioimaging. Taken together, these results demonstrate that erythrocyte membrane derived nanoparticles can be suggested as a biocompatible and spectrally validated nanoimaging platform, supporting their potential utility in bioimaging applications.