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<p>Effective skin tissue regeneration remains a clinical challenge, particularly in chronic and infected wounds, which require multifunctional dressings capable of combining physical protection, antimicrobial activity, and cellular stimulation. In this study, we report the development and characterization of electrospun poly(ε-caprolactone) (PCL) membranes incorporated with copaiba oleoresin (CO), a traditional Amazonian bioactive agent known for its wound-healing and anti-inflammatory properties but still underexplored in nanofiber-based systems. Gas chromatography–mass spectrometry (GC–MS) confirmed the predominance of sesquiterpenes in CO, mainly β-caryophyllene (34.61%), γ-muurolene (10.41%), and α-copaene (6.95%). The incorporation of CO significantly modulated PCL properties, increasing fiber diameters from 263 ± 48&#xa0;nm to 426 ± 120&#xa0;nm, reducing crystallinity, and decreasing melting and crystallization temperatures. Functional assays revealed enhanced surface hydrophilicity (contact angle reduced to 74.36°), liquid uptake up to 120% of the initial weight, and accelerated in vitro degradation (23% mass loss at 56 days). PCL/CO membranes exhibited strong antimicrobial activity against <i>Staphylococcus aureus</i> (&gt; 90% inhibition), while maintaining mechanical integrity (3–5&#xa0;MPa tensile strength) and excellent biocompatibility (&gt; 90% fibroblast viability after 72&#xa0;h). Negative zeta potential values (–20 to − 30 mV) further indicated potential for antimicrobial ion adsorption. Collectively, these findings demonstrate that PCL/CO electrospun nanofibers synergistically combine structural stability, antimicrobial functionality, and cytocompatibility, highlighting their promise as sustainable, bioactive wound dressings inspired by Amazonian biodiversity.</p>

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Electrospun Nanofibers from poly(ε-caprolactone) with Copaiba (Copaifera sp.) Oleoresin as an Advanced Healer

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月19日

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  • Andrey Marcos Pinho da Silva,
  • Marcel Afonso Provenzi,
  • Marília Miotto,
  • Susane Lopes,
  • Marcelo Maraschin,
  • Claudia Merlini

摘要

Effective skin tissue regeneration remains a clinical challenge, particularly in chronic and infected wounds, which require multifunctional dressings capable of combining physical protection, antimicrobial activity, and cellular stimulation. In this study, we report the development and characterization of electrospun poly(ε-caprolactone) (PCL) membranes incorporated with copaiba oleoresin (CO), a traditional Amazonian bioactive agent known for its wound-healing and anti-inflammatory properties but still underexplored in nanofiber-based systems. Gas chromatography–mass spectrometry (GC–MS) confirmed the predominance of sesquiterpenes in CO, mainly β-caryophyllene (34.61%), γ-muurolene (10.41%), and α-copaene (6.95%). The incorporation of CO significantly modulated PCL properties, increasing fiber diameters from 263 ± 48 nm to 426 ± 120 nm, reducing crystallinity, and decreasing melting and crystallization temperatures. Functional assays revealed enhanced surface hydrophilicity (contact angle reduced to 74.36°), liquid uptake up to 120% of the initial weight, and accelerated in vitro degradation (23% mass loss at 56 days). PCL/CO membranes exhibited strong antimicrobial activity against Staphylococcus aureus (> 90% inhibition), while maintaining mechanical integrity (3–5 MPa tensile strength) and excellent biocompatibility (> 90% fibroblast viability after 72 h). Negative zeta potential values (–20 to − 30 mV) further indicated potential for antimicrobial ion adsorption. Collectively, these findings demonstrate that PCL/CO electrospun nanofibers synergistically combine structural stability, antimicrobial functionality, and cytocompatibility, highlighting their promise as sustainable, bioactive wound dressings inspired by Amazonian biodiversity.