Scholarlink

<p> Purpose: Placental extract exhibits multiple therapeutic properties, including anti-inflammatory, antimicrobial, anti-apoptotic, anti-tumor, antioxidant, immunomodulatory, and regenerative effects. Materials and methods: Human chorionic villi were homogenized and enzymatically digested, followed by sequential centrifugation and lyophilization to obtain the placental extract. Human placental extract (HPE) and its derived nanovesicles (HPE-derived nanovesicles) were assessed for morphological, structural, chemical, biological, and biocompatibility characteristics. Results: HPE-derived nanovesicles exhibited hollow spheres surrounded by lipid bilayers, with uniform distribution and high structural homogeneity. Scanning Electron Microscopy and Transmission Electron Microscopy analyses confirmed homogeneous nanovesicles with uniform distribution, low aggregation, and sizes ranging from 40 to 160&#xa0;nm. Dynamic Light Scattering analysis revealed dominant populations of nanovesicle averaging 115.1–121.7&#xa0;nm (Mode:112.1–124.5&#xa0;nm) across three repetitions. The polydispersity index (0.223, 0.319, and 0.349), Z-average (92.8&#xa0;nm, 93&#xa0;nm, and 102.9&#xa0;nm), zeta potential (<b>-</b>34mV, -38.3mV, and − 37.9mV), and electrostatic stability (<b>-</b>0.000264&#xa0;cm²/V, 0.000298&#xa0;cm²/V, and 0.000295&#xa0;cm²/V) in three repetitions confirmed uniformity, strong colloidal stability of nanovesicles, suggesting that the vesicles remain well-dispersed and resistant to fluctuations or aggregation over time. Immunoblotting confirmed the expression of exosomal markers CD9, CD63, and CD81, demonstrating reliability and reproducibility. Total protein concentration averaged 0.6128&#xa0;mg/ml, while superoxide dismutase activity reached 34 ± 0.3U/mg, as determined by Bicinchoninic Acid and colorimetric assays. Fourier Transform Infrared Spectroscopy revealed distinct absorption bands, supporting the complex biochemical composition of the nanovesicles. Microbiological testing verified sterility, and in vivo irritation and sensitization assay showed no edema, erythema, or eschar formation, consistent with ISO 11737-2 and ISO 10,993. Conclusion: HPE-derived nanovesicles are stable, reproducible, biocompatible, and suitable for therapeutic and drug delivery applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comprehensive Characterization of Human Placental Extract-Derived Nanovesicles: Insight into Structural, Morphological, Chemical, Biological, and Biocompatible Properties

  • 期刊论文

  • 发表日期：

    2026年2月16日

查看全文
  • Hadiseh Rajab Zadeh,
  • Banafsheh Heidari,
  • Hanieh Jafary,
  • Kavosh Zandsalimi,
  • Nazanin Akbari

摘要

Purpose: Placental extract exhibits multiple therapeutic properties, including anti-inflammatory, antimicrobial, anti-apoptotic, anti-tumor, antioxidant, immunomodulatory, and regenerative effects. Materials and methods: Human chorionic villi were homogenized and enzymatically digested, followed by sequential centrifugation and lyophilization to obtain the placental extract. Human placental extract (HPE) and its derived nanovesicles (HPE-derived nanovesicles) were assessed for morphological, structural, chemical, biological, and biocompatibility characteristics. Results: HPE-derived nanovesicles exhibited hollow spheres surrounded by lipid bilayers, with uniform distribution and high structural homogeneity. Scanning Electron Microscopy and Transmission Electron Microscopy analyses confirmed homogeneous nanovesicles with uniform distribution, low aggregation, and sizes ranging from 40 to 160 nm. Dynamic Light Scattering analysis revealed dominant populations of nanovesicle averaging 115.1–121.7 nm (Mode:112.1–124.5 nm) across three repetitions. The polydispersity index (0.223, 0.319, and 0.349), Z-average (92.8 nm, 93 nm, and 102.9 nm), zeta potential (-34mV, -38.3mV, and − 37.9mV), and electrostatic stability (-0.000264 cm²/V, 0.000298 cm²/V, and 0.000295 cm²/V) in three repetitions confirmed uniformity, strong colloidal stability of nanovesicles, suggesting that the vesicles remain well-dispersed and resistant to fluctuations or aggregation over time. Immunoblotting confirmed the expression of exosomal markers CD9, CD63, and CD81, demonstrating reliability and reproducibility. Total protein concentration averaged 0.6128 mg/ml, while superoxide dismutase activity reached 34 ± 0.3U/mg, as determined by Bicinchoninic Acid and colorimetric assays. Fourier Transform Infrared Spectroscopy revealed distinct absorption bands, supporting the complex biochemical composition of the nanovesicles. Microbiological testing verified sterility, and in vivo irritation and sensitization assay showed no edema, erythema, or eschar formation, consistent with ISO 11737-2 and ISO 10,993. Conclusion: HPE-derived nanovesicles are stable, reproducible, biocompatible, and suitable for therapeutic and drug delivery applications.