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<p>The prevalence of Multi-Drugresistant (MDR) <i>Escherichia coli</i> in domestic sewage, necessitates the development of novel alternatives to conventional antibiotics. This study aimed to isolate and characterize MDR <i>E. coli</i> strains from domestic drainage water in Tenali, Andhra Pradesh, India. The current study investigated the antibacterial efficacy of nanocurcumin against MDR <i>E. coli</i> strains isolated from domestic drainage water. Ten samples were collected and screened for <i>E. coli</i> using EMB agar, Gram staining, motility and standard biochemical assays were performed. The isolated strains were subjected to antimicrobial susceptibility testing using the disc diffusion method. Curcumin was extracted from <i>Curcuma longa</i> and nanocurcumin was synthesized via physiochemical method, characterized using FTIR, SEM and XRD. All tested samples (100%) were found to be contaminated with MDR <i>E. coli.</i> Among these, resistance to cefixime was detected in 90% of the isolates, followed by tetracycline (70%) and ampicillin (50%), 40% of the isolates were resistant to kanamycin and amikacin, while ciprofloxacin, penicillin and chloramphenicol resistance was detected in 30% of the isolates. Nanocurcumin showed functional groups and partial crystallinity, with a spherical and polydisperse morphology ranging from 70 to 150&#xa0;nm in size. Antimicrobial testing demonstrated inhibition zones of 18 ± 0.54 – 25.1 ± 0.96&#xa0;mm, comparable to those of standard antibiotics. In conclusion, nanocurcumin exhibited significant antibacterial activity against MDR <i>E. coli</i> isolated from domestic drainage wastewater, highlighting its promise as a sustainable, natural antimicrobial agent for environmental applications. Further research is needed to evaluate its long-term efficacy, ecological safety and feasibility for field-scale implementation.</p> Graphical Abstract <p></p>

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Antimicrobial Activity of Nanocurcumin Against Multidrug-Resistant Escherichia coli Isolated from Domestic Drainage Water

  • 期刊论文

  • 发表日期：

    2026年1月16日

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  • Vallayyachari Kommoju,
  • Nikitha Priya Atyala,
  • Yaswanth Naveen Kumar Alla,
  • Syam Babu Davuluri,
  • Charles David

摘要

The prevalence of Multi-Drugresistant (MDR) Escherichia coli in domestic sewage, necessitates the development of novel alternatives to conventional antibiotics. This study aimed to isolate and characterize MDR E. coli strains from domestic drainage water in Tenali, Andhra Pradesh, India. The current study investigated the antibacterial efficacy of nanocurcumin against MDR E. coli strains isolated from domestic drainage water. Ten samples were collected and screened for E. coli using EMB agar, Gram staining, motility and standard biochemical assays were performed. The isolated strains were subjected to antimicrobial susceptibility testing using the disc diffusion method. Curcumin was extracted from Curcuma longa and nanocurcumin was synthesized via physiochemical method, characterized using FTIR, SEM and XRD. All tested samples (100%) were found to be contaminated with MDR E. coli. Among these, resistance to cefixime was detected in 90% of the isolates, followed by tetracycline (70%) and ampicillin (50%), 40% of the isolates were resistant to kanamycin and amikacin, while ciprofloxacin, penicillin and chloramphenicol resistance was detected in 30% of the isolates. Nanocurcumin showed functional groups and partial crystallinity, with a spherical and polydisperse morphology ranging from 70 to 150 nm in size. Antimicrobial testing demonstrated inhibition zones of 18 ± 0.54 – 25.1 ± 0.96 mm, comparable to those of standard antibiotics. In conclusion, nanocurcumin exhibited significant antibacterial activity against MDR E. coli isolated from domestic drainage wastewater, highlighting its promise as a sustainable, natural antimicrobial agent for environmental applications. Further research is needed to evaluate its long-term efficacy, ecological safety and feasibility for field-scale implementation.

Graphical Abstract