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<p>The current study investigates the antidiabetic potential of silver nanoparticles synthesized using <i>Lentinula edodes</i> (shiitake mushroom) extract, highlighting the green synthesis method, which makes use of the bioactive potential of natural phytocompounds. The biosynthesized silver nanoparticles were characterized using UV–Visible spectrophotometry, Fourier-transform infrared spectroscopy, X-ray diffraction, dynamic light scattering and field emission scanning electron microscopy to link their formation, stability, size distribution, surface morphology and crystalline nature. <i>In vitro</i> antioxidant potential of silver nanoparticles was tested using (2,2 diphenyl-1-picrylhydrazyl) radical scavenging assays. Α-amylase inhibitory assay revealed significant antidiabetic activity. <i>In silico</i> studies like molecular docking were performed to explore the critical phytocompounds from the <i>L. edodes</i> extract as ligands and target proteins (α-glucosidase, α-amylase) on glucose metabolism, and have shown strong binding affinities and possible mechanisms of action. Alloxan-induced diabetic Zebrafish were used for <i>in vivo</i> antidiabetic efficacy evaluation. Liver histoarchitecture was restored, and blood glucose level was significantly reduced in treatment with <i>L. edodes</i>-mediated silver nanoparticles. Overall, the findings indicate the potential of a combined effect of mushroom phytocompounds and silver nanoparticles in managing diabetes. This integrative work highlights the potential of using <i>L. edodes</i>-mediated silver nanoparticles as new-generation antidiabetic therapeutics with the development of <i>in vitro</i>, <i>in vivo</i>, and <i>in silico</i> study approaches.</p> Graphical Abstract <p></p>

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In Silico, In Vitro, and In Vivo Anti-Diabetic Potential of Mushroom Mediated Silver Nanoparticles Using Lentinula edodes Extract in Zebrafish Model

  • 期刊论文

  • 发表日期：

    2025年9月17日

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  • Logeshwari P.,
  • Architha Vijayalakshmi,
  • S. Hemalatha

摘要

The current study investigates the antidiabetic potential of silver nanoparticles synthesized using Lentinula edodes (shiitake mushroom) extract, highlighting the green synthesis method, which makes use of the bioactive potential of natural phytocompounds. The biosynthesized silver nanoparticles were characterized using UV–Visible spectrophotometry, Fourier-transform infrared spectroscopy, X-ray diffraction, dynamic light scattering and field emission scanning electron microscopy to link their formation, stability, size distribution, surface morphology and crystalline nature. In vitro antioxidant potential of silver nanoparticles was tested using (2,2 diphenyl-1-picrylhydrazyl) radical scavenging assays. Α-amylase inhibitory assay revealed significant antidiabetic activity. In silico studies like molecular docking were performed to explore the critical phytocompounds from the L. edodes extract as ligands and target proteins (α-glucosidase, α-amylase) on glucose metabolism, and have shown strong binding affinities and possible mechanisms of action. Alloxan-induced diabetic Zebrafish were used for in vivo antidiabetic efficacy evaluation. Liver histoarchitecture was restored, and blood glucose level was significantly reduced in treatment with L. edodes-mediated silver nanoparticles. Overall, the findings indicate the potential of a combined effect of mushroom phytocompounds and silver nanoparticles in managing diabetes. This integrative work highlights the potential of using L. edodes-mediated silver nanoparticles as new-generation antidiabetic therapeutics with the development of in vitro, in vivo, and in silico study approaches.

Graphical Abstract