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<p>This work presents a novel multi-mode method for the colorimetric detection of dopamine (DA), uric acid (UA), and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) using zeolitic imidazole framework (ZIF-L-Co) nanozyme. The nanozyme synthesized through an ultrasound-assisted co-precipitation method showcases high peroxidase-like activity, rich catalytic metal sites, and stability in high temperatures and water. Dynamic light scattering (DLS) measured a Zeta potential of 25.5&#xa0;mV and particle size of 2037 d.nm, while SEM analysis confirmed its 2D structure (4–5&#xa0;µm length and 1.5–1.8&#xa0;µm width). The functional validation of the nanozyme was provided by FTIR-ATR, UV–VIS spectroscopy, XRD, and XPS. The elemental composition is used to confirm the catalytic activity of Co-based ZIF-L-Co nanozyme validation by XPS. The nanozyme catalyzed the TMB to oxTMB (blue) by decomposing H<sub>2</sub>O<sub>2</sub>. The optimized parameter enabled the detection of H<sub>2</sub>O<sub>2</sub> with a limit of detection (LOD) of 1.3013&#xa0;µM and a quantification limit (LOQ) of 3.9434&#xa0;µM (linear range of 1–20&#xa0;µM, R<sup>2</sup> = 0.9932). For DA, it achieved a LOD of 2.32&#xa0;µM and LOQ of 3.633&#xa0;µM (linear range of 2.0–10&#xa0;µM, R<sup>2</sup> = 0.991), while UA sensing revealed two calibration curves (5–80&#xa0;µM and 80–300&#xa0;µM) with LOD of 15.65&#xa0;µM and LOQ of 47.42&#xa0;µM. This work highlights the potential of MOF in developing a selective, sensitive multimode biosensor for medical diagnostics.</p> Graphical Abstract <p>Schematic representation of high peroxidase ZIF-L-Co nanozyme for multi-mode quick optical colorimetric detection of H<sub>2</sub>O<sub>2</sub>, Uric Acid, and Dopamine.</p> <p></p>

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Rapid Multi-Mode Optical Colorimetric detection of H2O2, Uric Acid, and Dopamine using Cobalt-Based 2D ZIF-L-Co Nanozyme

  • 期刊论文

  • 发表日期：

    2025年7月10日

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  • Santosh Sethi,
  • V. K. Rathod

摘要

This work presents a novel multi-mode method for the colorimetric detection of dopamine (DA), uric acid (UA), and hydrogen peroxide (H2O2) using zeolitic imidazole framework (ZIF-L-Co) nanozyme. The nanozyme synthesized through an ultrasound-assisted co-precipitation method showcases high peroxidase-like activity, rich catalytic metal sites, and stability in high temperatures and water. Dynamic light scattering (DLS) measured a Zeta potential of 25.5 mV and particle size of 2037 d.nm, while SEM analysis confirmed its 2D structure (4–5 µm length and 1.5–1.8 µm width). The functional validation of the nanozyme was provided by FTIR-ATR, UV–VIS spectroscopy, XRD, and XPS. The elemental composition is used to confirm the catalytic activity of Co-based ZIF-L-Co nanozyme validation by XPS. The nanozyme catalyzed the TMB to oxTMB (blue) by decomposing H2O2. The optimized parameter enabled the detection of H2O2 with a limit of detection (LOD) of 1.3013 µM and a quantification limit (LOQ) of 3.9434 µM (linear range of 1–20 µM, R2 = 0.9932). For DA, it achieved a LOD of 2.32 µM and LOQ of 3.633 µM (linear range of 2.0–10 µM, R2 = 0.991), while UA sensing revealed two calibration curves (5–80 µM and 80–300 µM) with LOD of 15.65 µM and LOQ of 47.42 µM. This work highlights the potential of MOF in developing a selective, sensitive multimode biosensor for medical diagnostics.

Graphical Abstract

Schematic representation of high peroxidase ZIF-L-Co nanozyme for multi-mode quick optical colorimetric detection of H2O2, Uric Acid, and Dopamine.