<p>Laccase is a kind of green biocatalyst which is widely distributed in nature. However, the application of free laccase was greatly limited due to the poor stability and low utilization. In order to prepare a carrier with abundant immobilized sites, This study designed a novel magnetic dendrimers (MPG-x) as an excellent carrier for immobilizing laccase. Fe₃O₄ was used as the core, and the desired MPG-x was obtained by modifying the surface of Fe₃O₄ with hyperbranched polyaminoamide (PAMAM) dendrimers. Due to its controllable generation number, this carrier can provide a relatively abundant number of surface binding sites for laccase immobilization. Then the immobilized laccase MPG-2@GA-Lac and MPG-2@Cu²⁺-Lac were prepared by glutaraldehyde crosslinking and copper chelating adsorption, respectively. The results showed that the loading of MPG-2@GA-Lac and MPG-2@Cu²⁺-Lac reached 48.9&#xa0;mg/g and 50.6&#xa0;mg/g respectively, and MPG-2@GA-Lac and MPG-2@Cu²⁺-Lac retained 74.4% and 108.1% of the initial free laccase activity, respectively. In addition, the two immobilized laccase showed better pH stability, temperature stability, thermal stability and storage stability than free laccase. The activation of Cu²⁺ in the active center of laccase made MPG-2@Cu²⁺ -Lac exhibit better stability. Methyl orange (MO) removal experiments showed that MPG-2@Cu²⁺-Lac exhibited higher MO removal efficiency (82.56%) over a wider pH and temperature range than MPG-2@GA-Lac (72.19%), and MPG-2@Cu²⁺-Lac maintained a MO removal efficiency of 66.18% after 6 cycles of operation. This study is expected to further expand the application of laccase in the degradation of dye wastewater.</p>