Scholarlink

<p>Chronic suppurative otitis media (CSOM), often due to multidrug-resistant (MDR) bacteria, is a serious therapeutic challenge with increasing antibiotic resistance. The current study investigates the antibacterial potential of silver nanoparticles (AgNPs) biosynthesized by <i>Lactobacillus acidophilus</i> (LA-AgNPs) and mixed Lactobacillus culture (ML-AgNPs) against MDR CSOM isolates, i.e., <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>, <i>Proteus mirabilis</i>, <i>Klebsiella pneumoniae</i>, and <i>Enterobacter aerogenes</i>. Nanoparticles were characterized by UV–Vis, XRD, AFM, FE-SEM, and FTIR, revealing their spherical, crystalline shapes with sizes of 6.4 ± 1.2 nm (LA-AgNPs) and 82.89 ± 12.5 nm (ML-AgNPs), stabilized by microbial metabolites. Agar well diffusion assays demonstrated inhibitory zones of 22.5–30 mm (ML-AgNPs) and 20–25 mm (LA-AgNPs) against Gram-negative pathogens, with MIC₉₀ values ranging from 4.8–77.5 µg/mL (ML-AgNPs) to 9.7–38.7 µg/mL (LA-AgNPs). LA-AgNPs reduced bacterial viability to 7.68–73.50% at concentrations up to 38.7 µg/mL, showing dose-dependent efficacy. These results affirm the potential of probiotic-derived AgNPs as biocompatible alternatives to traditional antibiotics for treating multidrug-resistant CSOM infections.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Lactobacillus acidophilus and Mixed Probiotic-Mediated Synthesis of Silver Nanoparticles: Antibacterial Efficacy Against Multidrug-Resistant Otopathogens

  • 期刊论文

  • 发表日期：

    2025年6月18日

查看全文
  • Ammar Algburi,
  • Rand M. Mubarak,
  • Tahseen H. Mubarak,
  • Sonia Fathi-karkan,
  • Abbas Rahdar,
  • Luiz Fernando Romanholo Ferreira

摘要

Chronic suppurative otitis media (CSOM), often due to multidrug-resistant (MDR) bacteria, is a serious therapeutic challenge with increasing antibiotic resistance. The current study investigates the antibacterial potential of silver nanoparticles (AgNPs) biosynthesized by Lactobacillus acidophilus (LA-AgNPs) and mixed Lactobacillus culture (ML-AgNPs) against MDR CSOM isolates, i.e., Pseudomonas aeruginosa, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae, and Enterobacter aerogenes. Nanoparticles were characterized by UV–Vis, XRD, AFM, FE-SEM, and FTIR, revealing their spherical, crystalline shapes with sizes of 6.4 ± 1.2 nm (LA-AgNPs) and 82.89 ± 12.5 nm (ML-AgNPs), stabilized by microbial metabolites. Agar well diffusion assays demonstrated inhibitory zones of 22.5–30 mm (ML-AgNPs) and 20–25 mm (LA-AgNPs) against Gram-negative pathogens, with MIC₉₀ values ranging from 4.8–77.5 µg/mL (ML-AgNPs) to 9.7–38.7 µg/mL (LA-AgNPs). LA-AgNPs reduced bacterial viability to 7.68–73.50% at concentrations up to 38.7 µg/mL, showing dose-dependent efficacy. These results affirm the potential of probiotic-derived AgNPs as biocompatible alternatives to traditional antibiotics for treating multidrug-resistant CSOM infections.

Graphical Abstract