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<p>Antimicrobial resistance (AMR) is a critical global health challenge, exacerbated by biofilm-forming pathogens such as <i>Staphylococcus aureus</i> and <i>Candida albicans</i>. Biofilms act as physical barriers, hindering antimicrobial penetration and promoting pathogen persistence. This study explores the potential of biogenic silver nanoparticles (AgNPs B) as a sustainable alternative to combat AMR and biofilm-associated infections. AgNPs B were evaluated in relation to antimicrobial and antibiofilm activities, comparing with chemically synthesized silver nanoparticles (AgNPs C). AgNPs B demonstrated superior efficacy against multidrug-resistant (MDR) pathogens, including methicillin-resistant <i>S. aureus</i> (MRSA) and MDR <i>Pseudomonas aeruginosa</i>. They inhibited up to 87% of biofilm formation and degraded 98% of preformed MRSA biofilms, as confirmed by scanning electron microscopy (SEM), which revealed drastic reductions in biofilm structure with AgNPs B but not with AgNPs C. Moreover, AgNPs B reduced biofilm formation in <i>C. albicans</i> by 64–69%. Regarding filamentous fungi, AgNPs B were effective against <i>Rhizopus stolonifer</i> and <i>Alternaria alternata</i>, but less effective against <i>Fusarium oxysporum</i> and <i>Aspergillus niger</i>, likely due to their robust cell walls. No relevant virucidal activity was observed against human adenovirus type 5. However, our findings highlight the promising antimicrobial and antibiofilm properties of AgNPs B, particularly against MDR pathogens, and underscore their potential for applications in healthcare settings, as these nanoparticles have previously demonstrated low cytotoxicity in fibroblast cells. Further studies are needed to explore their broader use, including as surface disinfectants, and to evaluate their long-term safety and efficacy.</p>

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Application of Biogenic Silver Nanoparticles for Microbial and Biofilm Control

  • 期刊论文

  • 发表日期：

    2025年5月30日

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  • Gabriela Pereira Chuy,
  • Beatriz Pereira Savi,
  • Catielen Paula Pavi,
  • Giovanna Amaral Filipe,
  • Diogo Robl,
  • Gislaine Fongaro,
  • William Leonardo da Silva,
  • Fabienne Antunes Ferreira

摘要

Antimicrobial resistance (AMR) is a critical global health challenge, exacerbated by biofilm-forming pathogens such as Staphylococcus aureus and Candida albicans. Biofilms act as physical barriers, hindering antimicrobial penetration and promoting pathogen persistence. This study explores the potential of biogenic silver nanoparticles (AgNPs B) as a sustainable alternative to combat AMR and biofilm-associated infections. AgNPs B were evaluated in relation to antimicrobial and antibiofilm activities, comparing with chemically synthesized silver nanoparticles (AgNPs C). AgNPs B demonstrated superior efficacy against multidrug-resistant (MDR) pathogens, including methicillin-resistant S. aureus (MRSA) and MDR Pseudomonas aeruginosa. They inhibited up to 87% of biofilm formation and degraded 98% of preformed MRSA biofilms, as confirmed by scanning electron microscopy (SEM), which revealed drastic reductions in biofilm structure with AgNPs B but not with AgNPs C. Moreover, AgNPs B reduced biofilm formation in C. albicans by 64–69%. Regarding filamentous fungi, AgNPs B were effective against Rhizopus stolonifer and Alternaria alternata, but less effective against Fusarium oxysporum and Aspergillus niger, likely due to their robust cell walls. No relevant virucidal activity was observed against human adenovirus type 5. However, our findings highlight the promising antimicrobial and antibiofilm properties of AgNPs B, particularly against MDR pathogens, and underscore their potential for applications in healthcare settings, as these nanoparticles have previously demonstrated low cytotoxicity in fibroblast cells. Further studies are needed to explore their broader use, including as surface disinfectants, and to evaluate their long-term safety and efficacy.