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<p>In this work, ZnO–Au nanohybrids (NHPs) were successfully synthesized using a straightforward two-step nonaqueous method. The XRD pattern of the ZnO–Au HNPs powder exhibited diffraction peaks corresponding to the hexagonal wurtzite structure of ZnO phase. Additional peaks associated with the gold nanoparticles were also identified. The crystallite size of the ZnO–Au NHPs was determined to be 13&#xa0;nm, confirming their nanoscale dimensions. The FTIR spectrum displayed an absorption band at 432&#xa0;cm<sup>−1</sup>, indicating the presence of Zn–O bonds. The morphological properties observed in SEM images revealed that ZnO–Au NHPs have a spherical shape, agglomerating into small and large grains well distributed throughout the sample, with the particle size of the prepared HNPs being approximately (80–95) nm. The antibacterial activity of various concentrations of ZnO–Au NHPs was evaluated, demonstrating strong efficacy against <i>E. coli</i> and <i>Staph.</i> Bacteria. The inhibition zone and antibacterial effectiveness increased from 17 to 20&#xa0;mm against <i>Staph.</i> bacteria and from 15 to 18&#xa0;mm against <i>E. coli</i> Bacteria as the hybrid nanoparticle concentrations rose from 250&#xa0;μg/ml to 1000&#xa0;μg/ml. The most significant activity was observed against <i>Staph.</i> Bacteria at a concentration of 1000&#xa0;μg/ml.</p>

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Synthesis of ZnO–Au Nanohybrids via a Nonaqueous, Straight Forward Method: Characterization and Evaluation of Their Antibacterial Activity

  • 期刊论文

  • 发表日期：

    2025年5月26日

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  • Fares A. Alkufy,
  • Sarah J. Torki,
  • Laith Hayder Al-Tamimi,
  • Ehsan M. Abbas,
  • Sarah A. Shaker,
  • Adel H. Omran Alkhayatt

摘要

In this work, ZnO–Au nanohybrids (NHPs) were successfully synthesized using a straightforward two-step nonaqueous method. The XRD pattern of the ZnO–Au HNPs powder exhibited diffraction peaks corresponding to the hexagonal wurtzite structure of ZnO phase. Additional peaks associated with the gold nanoparticles were also identified. The crystallite size of the ZnO–Au NHPs was determined to be 13 nm, confirming their nanoscale dimensions. The FTIR spectrum displayed an absorption band at 432 cm−1, indicating the presence of Zn–O bonds. The morphological properties observed in SEM images revealed that ZnO–Au NHPs have a spherical shape, agglomerating into small and large grains well distributed throughout the sample, with the particle size of the prepared HNPs being approximately (80–95) nm. The antibacterial activity of various concentrations of ZnO–Au NHPs was evaluated, demonstrating strong efficacy against E. coli and Staph. Bacteria. The inhibition zone and antibacterial effectiveness increased from 17 to 20 mm against Staph. bacteria and from 15 to 18 mm against E. coli Bacteria as the hybrid nanoparticle concentrations rose from 250 μg/ml to 1000 μg/ml. The most significant activity was observed against Staph. Bacteria at a concentration of 1000 μg/ml.