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<p>The antioxidant activity of 3 wt% copper-doped hydroxyapatite (Cu–HAP) was evaluated using the DPPH radical-scavenging assay. Cu–HAP was synthesised by the sol–gel technique, and its structural properties were characterised using energy-dispersive X-ray spectroscopy (EDX), field-emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The XRD analysis reveals that the Ca atom is substituted by the Cu atom in pure HAP, decreasing the crystallite size. FESEM analysis confirms grain size reduction from 134.99 to 72.69&#xa0;nm. Better apatite formation in SBF solution is observed in Cu-HAP in comparison to pure HAP using FESEM. The DPPH test showed that samples displayed significant DPPH radical scavenging capability in a dose-dependent fashion. A plausible mechanism of the antioxidation activity of Cu-HAP with standard DPPH is discussed. It is observed that Cu-doped HAP samples showed better antioxidant activity than pure HAP, excelling in scavenging ability at specific concentrations. Improved IC<sub>50</sub> value of 25.03 for Cu-HAP sample is measured in comparison to 35 for pure HAP. In vitro cytotoxicity assay with L929 cell lines shows better cell viability at lower concentrations for the Cu-HAP in comparison to pure HAP.</p>

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High Antioxidant Activity, Enhanced Cytocompatibility, Improved Apatite Formation, and Moderate Thrombogenicity of Copper-Doped Hydroxyapatite Attributed to Structural Changes Analysed Using EDX, FTIR, XRD, and FESEM

  • 期刊论文

  • 发表日期：

    2025年5月19日

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  • Ranbir Kumar,
  • Ullas Topno,
  • Deep Shikha,
  • Smit Anand,
  • Sanjay Kumar Sinha,
  • Paresh Kumar Mohanty,
  • Sanjay Mhaske

摘要

The antioxidant activity of 3 wt% copper-doped hydroxyapatite (Cu–HAP) was evaluated using the DPPH radical-scavenging assay. Cu–HAP was synthesised by the sol–gel technique, and its structural properties were characterised using energy-dispersive X-ray spectroscopy (EDX), field-emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The XRD analysis reveals that the Ca atom is substituted by the Cu atom in pure HAP, decreasing the crystallite size. FESEM analysis confirms grain size reduction from 134.99 to 72.69 nm. Better apatite formation in SBF solution is observed in Cu-HAP in comparison to pure HAP using FESEM. The DPPH test showed that samples displayed significant DPPH radical scavenging capability in a dose-dependent fashion. A plausible mechanism of the antioxidation activity of Cu-HAP with standard DPPH is discussed. It is observed that Cu-doped HAP samples showed better antioxidant activity than pure HAP, excelling in scavenging ability at specific concentrations. Improved IC50 value of 25.03 for Cu-HAP sample is measured in comparison to 35 for pure HAP. In vitro cytotoxicity assay with L929 cell lines shows better cell viability at lower concentrations for the Cu-HAP in comparison to pure HAP.