Scholarlink

<p>Despite significant advancements in the field of cancer treatment, existing anticancer drugs, due to their inability to differentiate between healthy and tumor cells, have considerable side effects that can sometimes challenge the treatment process. Among malignant tumors originating from epithelial tissues, breast cancer is the most prevalent type among women. It is recognized as the most common cancer and the fifth leading cause of death in Iranian women. Recent progress in nanobiotechnology and the utilization of the unique properties of nanoparticles for targeted delivery of anticancer agents have positioned this field as an innovative approach to cancer treatment. This research aimed to deliver two anticancer agents, gemcitabine and quercetin, to breast cancer cells using aptamer-targeted single wall carbon nanotubes (SWCNTs). To this end, a drug delivery system based on SWCNTs was designed, in which both the drugs and the aptamer were attached to the carbon nanotubes through π-π bonds. Initially, the successful attachment of the aptamer as a targeting agent to the carbon nanotubes as a drug carrier was confirmed using CD, FTIR, UV–Vis, and Raman spectroscopy. Subsequently, the loading of gemcitabine and quercetin onto aptamer-functionalized carbon nanotubes and carbon nanotubes without aptamers was investigated using UV–Vis and FTIR spectroscopy. The results indicated that the amount of gemcitabine and quercetin loaded in the aptamer-targeted system was 12% and 13%, respectively, while in the non-targeted drug system, it was 7% and 6%, respectively. Additionally, the drug release in both systems was biphasic, with an initial burst release within the first two hours followed by a slower release rate. The presence of the aptamer in the system led to increased drug loading and slower drug release, and elemental analysis also confirmed the presence of elements corresponding to the drugs and aptamer in the nanosystem. Finally, cellular studies on the MCF-7 cell line demonstrated that the targeted nanosystem exhibited higher efficacy in killing cancer cells within 48 h compared to other groups.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Novel Tubular SWCNT/Aptamer Nanodelivery Smart System for Targeted Breast Cancer Treatment and Physico Chemical Study

  • 期刊论文

  • 发表日期：

    2025年5月19日

查看全文
  • Zahra Meghdari,
  • Aboulfazl Mirzapoor,
  • Bijan Ranjbar

摘要

Despite significant advancements in the field of cancer treatment, existing anticancer drugs, due to their inability to differentiate between healthy and tumor cells, have considerable side effects that can sometimes challenge the treatment process. Among malignant tumors originating from epithelial tissues, breast cancer is the most prevalent type among women. It is recognized as the most common cancer and the fifth leading cause of death in Iranian women. Recent progress in nanobiotechnology and the utilization of the unique properties of nanoparticles for targeted delivery of anticancer agents have positioned this field as an innovative approach to cancer treatment. This research aimed to deliver two anticancer agents, gemcitabine and quercetin, to breast cancer cells using aptamer-targeted single wall carbon nanotubes (SWCNTs). To this end, a drug delivery system based on SWCNTs was designed, in which both the drugs and the aptamer were attached to the carbon nanotubes through π-π bonds. Initially, the successful attachment of the aptamer as a targeting agent to the carbon nanotubes as a drug carrier was confirmed using CD, FTIR, UV–Vis, and Raman spectroscopy. Subsequently, the loading of gemcitabine and quercetin onto aptamer-functionalized carbon nanotubes and carbon nanotubes without aptamers was investigated using UV–Vis and FTIR spectroscopy. The results indicated that the amount of gemcitabine and quercetin loaded in the aptamer-targeted system was 12% and 13%, respectively, while in the non-targeted drug system, it was 7% and 6%, respectively. Additionally, the drug release in both systems was biphasic, with an initial burst release within the first two hours followed by a slower release rate. The presence of the aptamer in the system led to increased drug loading and slower drug release, and elemental analysis also confirmed the presence of elements corresponding to the drugs and aptamer in the nanosystem. Finally, cellular studies on the MCF-7 cell line demonstrated that the targeted nanosystem exhibited higher efficacy in killing cancer cells within 48 h compared to other groups.

Graphical Abstract