<p>Nanopillars on insect wings have been identified to possess bactericidal properties. Nanopillars are bactericidal only to those bacteria that are attached to it. Our previous study analyzed the viability of <i>Pseudomonas aeruginosa</i> PAO1 and ATCC 9027 on the nanopillar topography of the dragonfly (<i>Pantala flavescens</i>) wing against flat control surfaces. <i>P. aeruginosa</i> PAO1 exhibited higher viability than ATCC 9027, as PAO1 restrained from attaching to the nanopillars, evading its bactericidal effect, unlike ATCC 9027. It was speculated that bacterial surface mechanosensing was a probable mechanism and that bacteria likely used quorum-sensing molecules such as Acyl Homoserine Lactones (AHLs) for communication. In the present study, two strains of <i>Pseudomonas</i> were allowed to interact with the control and the wing separately, and AHLs were extracted. The AHLs analyzed using GC-EI/MS facilitated the identification of key AHLs involved in bacterial attachment and biofilm formation such as 3-oxo-C₁₀₂-HSL, 3-oxo-C₁₂-HSL, and C₄-HSL. Besides, few other AHLs that were specifically expressed such as C₉-HSL, 3-OH-C₁₁-HSL, 3-OH-C₁₃-HSL, and 3-OH-C₁₅-HSL were likely responsible for higher bacterial attachment. The roles and implications of these AHLs could be further explored to facilitate attachment of <i>P. aeruginosa</i> strains onto the nanopillar topography to enhance its bactericidal efficiency.</p> Graphical Abstract <p></p>