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<p>Zika virus (ZIKV) infection, a global public health emergency, is associated with microcephaly in newborns and Guillain-Barré syndrome in adults. However, there is currently no vaccine for Zika disease, and existing detection assays for this infection still require improvements in practicality, analysis time, and cross-reactivity with other arboviruses. Quantum dots (QDs) have stood out as valuable nanotools for fluorescence-based assays due to their exceptional brightness, resistance to photobleaching, and chemically active surfaces for conjugation. In this study, we developed a conjugate consisting of anti-ZIKV envelope protein monoclonal antibodies (anti-EP ZIKV) covalently bonded to QDs. The nanosystem was then evaluated in a capture fluoroimmunoassay, using microplates, for direct sensing of the EP (ZIKV). Results showed that the conjugate effectively detected the EP (ZIKV) individually. The fluorescence-based platform also identified ZIKV in complex biological samples within a short response time, exhibiting differentiation from a mixture of four dengue virus (DENV) serotypes. The conjugate showed effectiveness after 15&#xa0;days of preparation, and its performance improved after 1&#xa0;year of storage. Therefore, the QDs-(anti-EP ZIKV) nanosystem, combined with a microplate-based assay, showed promise for being applied in the detection of ZIKV infection during the viremic phase, stimulating the use of the conjugate in other fluorescence-based biosensing platforms.</p>

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Quantum Dot-Antibody Conjugates in a Direct Fluoroimmunoassay for Sensing the Envelope Protein of the Zika Virus

  • 期刊论文

  • 发表日期：

    2025年5月19日

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  • Jéssika F. F. Ribeiro,
  • Rennan R. M. Lima,
  • Claudilene R. Chaves,
  • Daniela S. Rosa,
  • Paulo E. Cabral Filho,
  • Adriana Fontes

摘要

Zika virus (ZIKV) infection, a global public health emergency, is associated with microcephaly in newborns and Guillain-Barré syndrome in adults. However, there is currently no vaccine for Zika disease, and existing detection assays for this infection still require improvements in practicality, analysis time, and cross-reactivity with other arboviruses. Quantum dots (QDs) have stood out as valuable nanotools for fluorescence-based assays due to their exceptional brightness, resistance to photobleaching, and chemically active surfaces for conjugation. In this study, we developed a conjugate consisting of anti-ZIKV envelope protein monoclonal antibodies (anti-EP ZIKV) covalently bonded to QDs. The nanosystem was then evaluated in a capture fluoroimmunoassay, using microplates, for direct sensing of the EP (ZIKV). Results showed that the conjugate effectively detected the EP (ZIKV) individually. The fluorescence-based platform also identified ZIKV in complex biological samples within a short response time, exhibiting differentiation from a mixture of four dengue virus (DENV) serotypes. The conjugate showed effectiveness after 15 days of preparation, and its performance improved after 1 year of storage. Therefore, the QDs-(anti-EP ZIKV) nanosystem, combined with a microplate-based assay, showed promise for being applied in the detection of ZIKV infection during the viremic phase, stimulating the use of the conjugate in other fluorescence-based biosensing platforms.