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<p>The study focused on the evaluation of antibiofilm and antimicrobial properties of biosurfactants synthesized using baker’s yeast, which was subsequently utilized as a stabilizing and reducing agent for the synthesis of ZnO nanoparticles. Characterization of biosurfactant-coated nanoparticles was done using UV, XRD, SEM, and FTIR spectroscopy. SEM images indicated the formation of nanoforest resulting in a higher aspect ratio. FTIR analysis of BS-coated ZnO NPs indicated characteristic ZnO stretching confirming the existence of ZnO nanoparticles, while biosurfactant FTIR spectra showed characteristic stretching vibrations, indicating the presence of hydrophobic and hydrophilic groups. The antibacterial effects of <i>Klebsiella pneumoniae</i> and <i>Pseudomonas aeruginosa</i> on cotton fabrics and paper disks showed varying results. The antibiofilm assay demonstrated maximum inhibition percentages of 96.20% for <i>K. pneumoniae</i> at a 5&#xa0;mg/mL biosurfactant concentration, 93.86% for <i>Staphylococcus aureus</i> at 3&#xa0;mg/mL, and 71.21% for <i>P. aeruginosa</i> at 5&#xa0;mg/mL. Comparatively, treatment with nanoparticles showed the highest inhibition percentages of 94.09% for <i>K. pneumoniae</i> at 5&#xa0;mg/mL, 95.62% for <i>S. aureus</i> at 1&#xa0;mg/mL, and 77.27% for <i>P. aeruginosa</i> at 5&#xa0;mg/mL. MIC assay results revealed maximum efficiency for both <i>P. aeruginosa</i> and <i>K. pneumoniae</i> compared to the control across five dilutions. The study incorporating various antibiotics with biosurfactant and nanoparticles at a concentration of 1&#xa0;mg/mL showed efficient results, particularly highlighting ceftazidime and cefoxitin when treated with biosurfactant and nanoparticles. Overall, this research demonstrates the effective antibiofilm properties of biosurfactant and biosurfactant-coated ZnO nanoparticles against various pathogenic microbes.</p> Graphical Abstract <p></p>

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Biofilm Inhibition and Antimicrobial Properties of Biosynthesized Biosurfactant and Biosurfactant-Coated ZnO Nanoparticles Using Baker’s Yeast (Saccharomyces cerevisiae)

  • 期刊论文

  • 发表日期：

    2025年2月4日

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  • Faaiza Naaz K.,
  • Aabid Hussain Shaik,
  • Lavanya Balaji,
  • Madhumalathi D.,
  • Salma Sultana,
  • Mohammed Rehaan Chandan

摘要

The study focused on the evaluation of antibiofilm and antimicrobial properties of biosurfactants synthesized using baker’s yeast, which was subsequently utilized as a stabilizing and reducing agent for the synthesis of ZnO nanoparticles. Characterization of biosurfactant-coated nanoparticles was done using UV, XRD, SEM, and FTIR spectroscopy. SEM images indicated the formation of nanoforest resulting in a higher aspect ratio. FTIR analysis of BS-coated ZnO NPs indicated characteristic ZnO stretching confirming the existence of ZnO nanoparticles, while biosurfactant FTIR spectra showed characteristic stretching vibrations, indicating the presence of hydrophobic and hydrophilic groups. The antibacterial effects of Klebsiella pneumoniae and Pseudomonas aeruginosa on cotton fabrics and paper disks showed varying results. The antibiofilm assay demonstrated maximum inhibition percentages of 96.20% for K. pneumoniae at a 5 mg/mL biosurfactant concentration, 93.86% for Staphylococcus aureus at 3 mg/mL, and 71.21% for P. aeruginosa at 5 mg/mL. Comparatively, treatment with nanoparticles showed the highest inhibition percentages of 94.09% for K. pneumoniae at 5 mg/mL, 95.62% for S. aureus at 1 mg/mL, and 77.27% for P. aeruginosa at 5 mg/mL. MIC assay results revealed maximum efficiency for both P. aeruginosa and K. pneumoniae compared to the control across five dilutions. The study incorporating various antibiotics with biosurfactant and nanoparticles at a concentration of 1 mg/mL showed efficient results, particularly highlighting ceftazidime and cefoxitin when treated with biosurfactant and nanoparticles. Overall, this research demonstrates the effective antibiofilm properties of biosurfactant and biosurfactant-coated ZnO nanoparticles against various pathogenic microbes.

Graphical Abstract