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<p>The use of a combination strategy, including many chemotherapeutic drugs, has been recognized as a way to decrease the dosage of drugs and achieve a more effective simultaneous therapeutic effect in pancreatic ductal adenocarcinoma (PDAC) treatment. In this study, we constructed a very effective nanocarrier that simultaneously encapsulates and releases two chemotherapeutic drugs. The nanocarrier is composed of poly(lactide-co-glycolide) (PLG) and mesoporous silica composite nanofibers (NFs), which allow for sustained release of the drugs. The study used amine-functionalized mesoporous silica nanoparticles to load the hydrophilic gemcitabine (GEM) and the hydrophobic drug mitoxantrone (MTX). These loaded nanoparticles were referred to as GEM@MSNPs and MTX@MSNPs, respectively. Subsequently, two types of drug-loaded mesoporous silica nanoparticles (MSNPs) were combined&#xa0;in the poly(lactic-co-glycolic acid) (PLG) to fabricate a fibrous structure using the combination electrospinning technique. This procedure yields a nanofibrous framework that contains two drugs, PLG/GEM@MSNPs/MTX@MSNPs. The composite nanofibers were examined using various methods, and the results indicated successful encapsulation of GEM@MSNPs/MTX@MSNPs into electrospun PLG nanofibers. In addition, as related to the nanofibers loaded with a single drug, the nanofibers composed of a combination of two drugs showed greater effectiveness in inhibiting the growth of PANC-1 PDAC. The PANC-1 cells’ morphological changes were examined by biochemical staining and flow cytometric analysis. The wound migration assay examined the cell migration ratio of the PANC-1 cells. The results of our study suggest that these composite nanofibrous scaffolds, loaded with two drugs, can potentially be used in PDAC treatment.</p>

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Fabrication of Gemcitabine and Mitoxantrone Loaded PLG/Mesoporous Silica Nanofibers: Investigation of In Vitro Drug Release and Anticancer Activity in Pancreatic Cancer Cells

  • 期刊论文

  • 发表日期：

    2025年1月3日

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  • Hui-min Wu,
  • Sheng-lan Wang,
  • Xin-xing Li,
  • Kai-xing Ai

摘要

The use of a combination strategy, including many chemotherapeutic drugs, has been recognized as a way to decrease the dosage of drugs and achieve a more effective simultaneous therapeutic effect in pancreatic ductal adenocarcinoma (PDAC) treatment. In this study, we constructed a very effective nanocarrier that simultaneously encapsulates and releases two chemotherapeutic drugs. The nanocarrier is composed of poly(lactide-co-glycolide) (PLG) and mesoporous silica composite nanofibers (NFs), which allow for sustained release of the drugs. The study used amine-functionalized mesoporous silica nanoparticles to load the hydrophilic gemcitabine (GEM) and the hydrophobic drug mitoxantrone (MTX). These loaded nanoparticles were referred to as GEM@MSNPs and MTX@MSNPs, respectively. Subsequently, two types of drug-loaded mesoporous silica nanoparticles (MSNPs) were combined in the poly(lactic-co-glycolic acid) (PLG) to fabricate a fibrous structure using the combination electrospinning technique. This procedure yields a nanofibrous framework that contains two drugs, PLG/GEM@MSNPs/MTX@MSNPs. The composite nanofibers were examined using various methods, and the results indicated successful encapsulation of GEM@MSNPs/MTX@MSNPs into electrospun PLG nanofibers. In addition, as related to the nanofibers loaded with a single drug, the nanofibers composed of a combination of two drugs showed greater effectiveness in inhibiting the growth of PANC-1 PDAC. The PANC-1 cells’ morphological changes were examined by biochemical staining and flow cytometric analysis. The wound migration assay examined the cell migration ratio of the PANC-1 cells. The results of our study suggest that these composite nanofibrous scaffolds, loaded with two drugs, can potentially be used in PDAC treatment.