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Background <p>Gallbladder cancer (GBC) is the most common biliary tract malignancy and a leading cause for mortality in the Asian sub-continent. The low survival rate is attributed to current invasive diagnostic methods and late-stage disease detection. The aim of this study was to estimate the potential value of miR 183_5p and 3651 as diagnostic blood-based biomarkers in GBC patients.</p> Methods <p>This single center observational study evaluates differential expression of miRNAs in GBC and normal gallbladder tissues via micro-array analysis. The level of selected oncogenic miRNAS were detected in 130 individuals comprising GBC patients, Gallbladder stone (GBS) and healthy controls by using qRT-PCR. The diagnostic value of miR-183-5p and 3651 in GBC was evaluated and compared with the tumor markers carcinoembryonic antigen (CEA) and carbohydrate antigen 19.9 (CA 19.9).</p> Results <p>As many as&#xa0;45 differentially expressed microRNAs (<i>p</i> &lt; 0.05) were determined, of which 23 were upregulated and 22 were down regulated. miR 183_5p (2.5-fold) and 3651 (5.6-fold) were significantly over expressed in GBC patients compared to controls (<i>p</i> &lt; 0.05). The area under the curve (AUC)&#xa0;value of miR 183_5p and 3651 for GBC diagnosis was 0.684 and 0.752, respectively, which was more valuable than those including CEA (0.617) and CA19.9 (0.718). </p> Conclusion <p>Study underscores ability of serum miRNAs 183_5p and 3651 as diagnostic biomarkers for early GBC detection.</p> Graphical Abstract <p></p>

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Clinical significance of circulatory microRNA-183_5p and 3651 in serum as novel potential diagnostic marker for gallbladder cancer

  • 期刊论文

  • 发表日期：

    2026年4月8日

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  • Deepika Verma,
  • Neelam Lohani,
  • Joginder Kumar,
  • Vivek Nayyar,
  • Payal Gupta,
  • S. S. Chauhan

摘要

Background

Gallbladder cancer (GBC) is the most common biliary tract malignancy and a leading cause for mortality in the Asian sub-continent. The low survival rate is attributed to current invasive diagnostic methods and late-stage disease detection. The aim of this study was to estimate the potential value of miR 183_5p and 3651 as diagnostic blood-based biomarkers in GBC patients.

Methods

This single center observational study evaluates differential expression of miRNAs in GBC and normal gallbladder tissues via micro-array analysis. The level of selected oncogenic miRNAS were detected in 130 individuals comprising GBC patients, Gallbladder stone (GBS) and healthy controls by using qRT-PCR. The diagnostic value of miR-183-5p and 3651 in GBC was evaluated and compared with the tumor markers carcinoembryonic antigen (CEA) and carbohydrate antigen 19.9 (CA 19.9).

Results

As many as 45 differentially expressed microRNAs (p < 0.05) were determined, of which 23 were upregulated and 22 were down regulated. miR 183_5p (2.5-fold) and 3651 (5.6-fold) were significantly over expressed in GBC patients compared to controls (p < 0.05). The area under the curve (AUC) value of miR 183_5p and 3651 for GBC diagnosis was 0.684 and 0.752, respectively, which was more valuable than those including CEA (0.617) and CA19.9 (0.718).

Conclusion

Study underscores ability of serum miRNAs 183_5p and 3651 as diagnostic biomarkers for early GBC detection.

Graphical Abstract