Background <p>This study aimed to discover the relationship between Angle Class II-deep overbite (ACII-DOB) and anterior disc displacement (ADD) of temporomandibular disorder (TMD).</p> Materials and Methods <p>This was a retrospective cross-sectional imaging study. Patients diagnosed with TMD accompanied with ACII-DOB were enrolled. Magnetic resonance imaging (MRI) and cone-beam computed tomography were applied in both joints.</p> Results <p>258 patients were included. 153 and 24 patients presented joint click and limited mouth opening, respectively. MRI revealed 120-side normal disc-condyle relationship (NDCR), 226-side ADD with reduction (ADDwR) and 166-side ADD without reduction (ADDwoR). 166-side ADDwoR contained 74-side osteoarthritis (OA). MRI also discovered 4-side other types of disc displacement, including 1-side exterior, 1-side interior, and 2-side posterior disc displacement. Excluding these 4 sides, 512-side joints were classified into NDCR group (<i>n</i> = 120), ADDwR group (<i>n</i> = 226), ADDwoR without OA group (<i>n</i> = 92), and ADDwoR with OA group (<i>n</i> = 74). Disc length gradually reduced in four groups. Disc-condyle distances were larger in two ADDwoR groups than that in ADDwR group. The values of anterior joint space (AJS)/posterior joint space were 0.86 ± 0.30, 1.98 ± 0.72, 1.78 ± 0.75, and 1.37 ± 0.70 in four groups, respectively.</p> Conclusion <p>ADD was associated with enlarged AJS which was observed in ACII-DOB patients derived from a clinic-based TMD cohort.</p>