Scholarlink

<p>In India, agriculture wastes (AWs) create a serious environmental issue due to lack of proper management practices. Microorganisms produce hydrolases that convert these AWs into economic resources thereby constituting sustainability. Chitinases are one of these hydrolases that degrade chitin, a significant component of fungal cell wall. Use of AWs for producing chitinase adds value, reduces production and handling cost. Therefore, in this study we isolated microbes from dead organic matter and characterized them for chitinase activity. Developed consortium of <i>Gongronella butleri</i> (BH) and <i>Paenibacillus lautus</i> (E1) was assessed for chitinase production using corn-cob via solid state fermentation. Chitinase production was upscaled, followed by enzyme extraction (3406 U/ml), purification (2298 U/ml) and characterization. The partially purified enzyme showed anti-fungal activity against <i>Alternaria</i> sp. (50.25%), <i>Rhizoctonia solani</i> (23.92%) and <i>Fusarium oxysporum</i> (12.12%). Partially purified chitinase tested for environmental implications showed 52% reduction in the fungal population of dump-yard site soil. Cost analysis revealed 88% reduction in production costs compared to synthetic media. These chitinases have recently attracted attention due to its significant role in agricultural field by solving plant-related problems. Thus, this study offers a promising approach for valorizing agro-industrial residues for production of value-added product. </p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Valorization of Corn Cob Via Mixed Chitinase Production Using Solid State Fermentation Technology

  • 期刊论文

  • 发表日期：

    2026年5月8日

查看全文
  • Arpit Acharya,
  • Satyam Rastogi,
  • Pradeep Kumar,
  • Varsha Dharmesh,
  • Shashank Kumar Mishra,
  • Suchi Srivastava

摘要

In India, agriculture wastes (AWs) create a serious environmental issue due to lack of proper management practices. Microorganisms produce hydrolases that convert these AWs into economic resources thereby constituting sustainability. Chitinases are one of these hydrolases that degrade chitin, a significant component of fungal cell wall. Use of AWs for producing chitinase adds value, reduces production and handling cost. Therefore, in this study we isolated microbes from dead organic matter and characterized them for chitinase activity. Developed consortium of Gongronella butleri (BH) and Paenibacillus lautus (E1) was assessed for chitinase production using corn-cob via solid state fermentation. Chitinase production was upscaled, followed by enzyme extraction (3406 U/ml), purification (2298 U/ml) and characterization. The partially purified enzyme showed anti-fungal activity against Alternaria sp. (50.25%), Rhizoctonia solani (23.92%) and Fusarium oxysporum (12.12%). Partially purified chitinase tested for environmental implications showed 52% reduction in the fungal population of dump-yard site soil. Cost analysis revealed 88% reduction in production costs compared to synthetic media. These chitinases have recently attracted attention due to its significant role in agricultural field by solving plant-related problems. Thus, this study offers a promising approach for valorizing agro-industrial residues for production of value-added product.

Graphical Abstract