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<p>White-rot fungal pretreatment of lignocellulose is promising for animal feed and biofuel production, but its industrial application has been limited by the need for strict substrate sterilization. This study optimized the initial pH in a pasteurization-based dual-hurdle system for solid-state fermentation of wheat straw by <i>Irpex lacteus</i>. Specifically, pasteurized wheat straw was fermented for 28&#xa0;days at initial pH values ranging from 2.5 to 6.5. The results indicated that pH 4.5 was optimal, achieving over 30% degradation of acid detergent lignin, comparable to the autoclaved control, while largely preserving cellulose. This treatment also gave the highest glucose yield after enzymatic saccharification, significantly higher than that of all other treatments. Microbial analysis via 16S rDNA sequencing revealed that the pH 4.5 imposed strong selective pressure on the bacterial community and markedly reduced bacterial diversity. While heat-resistant <i>Bacilli</i> dominated at pH 6.5, pH 4.5 resulted in a highly simplified community dominated by the acid-tolerant genus <i>Pseudomonas.</i> These findings indicate that initial pH 4.5 can effectively reshape the bacterial community and support fungal delignification under a low-cost pasteurization-based system.</p> Graphic Abstract <p></p>

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Pasteurization Combined with Initial pH Adjustment Selectively Suppresses Bacterial Competitors and Promotes Fungal Delignification of Wheat Straw

  • 期刊论文

  • 发表日期：

    2026年5月2日

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  • Dongze Niu,
  • Xuanhe Chen,
  • Taoli Huhe,
  • Jianjun Ren,
  • Ruling Yuan

摘要

White-rot fungal pretreatment of lignocellulose is promising for animal feed and biofuel production, but its industrial application has been limited by the need for strict substrate sterilization. This study optimized the initial pH in a pasteurization-based dual-hurdle system for solid-state fermentation of wheat straw by Irpex lacteus. Specifically, pasteurized wheat straw was fermented for 28 days at initial pH values ranging from 2.5 to 6.5. The results indicated that pH 4.5 was optimal, achieving over 30% degradation of acid detergent lignin, comparable to the autoclaved control, while largely preserving cellulose. This treatment also gave the highest glucose yield after enzymatic saccharification, significantly higher than that of all other treatments. Microbial analysis via 16S rDNA sequencing revealed that the pH 4.5 imposed strong selective pressure on the bacterial community and markedly reduced bacterial diversity. While heat-resistant Bacilli dominated at pH 6.5, pH 4.5 resulted in a highly simplified community dominated by the acid-tolerant genus Pseudomonas. These findings indicate that initial pH 4.5 can effectively reshape the bacterial community and support fungal delignification under a low-cost pasteurization-based system.

Graphic Abstract