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<p>The wastewater from Baijiu production has the characteristics of high COD value, pollution and treatment cost, but it also contains rich nutrients and has the value of reuse. In this study, these wastewaters were used as raw materials to prepare for the development of functional liquid agricultural microbial agents with phosphate solubilizing properties and rich in a variety of plant probiotics. In this study, the phosphorus solubilization characteristics of <i>Acinetobacter</i> S1 in five different wastewaters were investigated by using the amount of dissolved phosphorus as the key evaluation index, and the most suitable wastewater type for fermentation was selected. In addition, GC–MS and LC–MS analysis techniques were used to comprehensively analyze the probiotic components in the fermentation broth, and the key phosphate-solubilizing metabolic pathways were preliminarily analyzed. It was found that the phosphate solubilization ability of <i>Acinetobacter</i> S1 to organic phosphorus and inorganic phosphorus was different in different wastewater. In the substrate containing 20% distiller’s grains extract (JZS), the available phosphorus concentration in <i>Acinetobacter</i> S1 fermentation broth was the highest (98.38 ± 1.20&#xa0;mg/L), followed by the substrate containing 100% yellow pot water (HDGS was 90.52 ± 0.10&#xa0;mg/L). With HDGS as the fermentation substrate, the phosphatase activity in the fermentation broth reached the highest at 36&#xa0;h. The fermentation broth of <i>Acinetobacter</i> S1 enriched a variety of plant probiotics, such as organic acids (such as tartaric acid, succinic acid and acetic acid), amino acids (aspartic acid, lysine, phenylalanine), alkaloids (such as indoleacetic acid) and other compounds. Metabolomic analysis revealed that the dual function of phosphate-solubilizing and growth-promoting of <i>Acinetobacter</i> S1 depends on the reprogramming of central carbon metabolism (especially the pentose phosphate pathway, citric acid cycle and glycolysis / gluconeogenesis) to provide energy and carbon skeleton, and the activation of secondary metabolism (especially the biosynthesis of multiple amino acids) to supply plant growth precursors. In summary, it is feasible to use <i>Acinetobacter</i> S1 fermentation to produce a fermentation broth with phosphorus-solubilizing ability and rich in various plant probiotics using HDGS as a substrate.</p> Graphical Abstract <p></p>

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Analysis of Phosphate-Solubilizing Characteristics and Metabolites of Acinetobacter in Baijiu Wastewater

  • 期刊论文

  • 发表日期：

    2026年4月13日

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  • Fengqing Wang,
  • Shuang Li,
  • Kai Fan,
  • Suyi Zhang,
  • Li Li,
  • Jiaping Yang,
  • Chuan Song

摘要

The wastewater from Baijiu production has the characteristics of high COD value, pollution and treatment cost, but it also contains rich nutrients and has the value of reuse. In this study, these wastewaters were used as raw materials to prepare for the development of functional liquid agricultural microbial agents with phosphate solubilizing properties and rich in a variety of plant probiotics. In this study, the phosphorus solubilization characteristics of Acinetobacter S1 in five different wastewaters were investigated by using the amount of dissolved phosphorus as the key evaluation index, and the most suitable wastewater type for fermentation was selected. In addition, GC–MS and LC–MS analysis techniques were used to comprehensively analyze the probiotic components in the fermentation broth, and the key phosphate-solubilizing metabolic pathways were preliminarily analyzed. It was found that the phosphate solubilization ability of Acinetobacter S1 to organic phosphorus and inorganic phosphorus was different in different wastewater. In the substrate containing 20% distiller’s grains extract (JZS), the available phosphorus concentration in Acinetobacter S1 fermentation broth was the highest (98.38 ± 1.20 mg/L), followed by the substrate containing 100% yellow pot water (HDGS was 90.52 ± 0.10 mg/L). With HDGS as the fermentation substrate, the phosphatase activity in the fermentation broth reached the highest at 36 h. The fermentation broth of Acinetobacter S1 enriched a variety of plant probiotics, such as organic acids (such as tartaric acid, succinic acid and acetic acid), amino acids (aspartic acid, lysine, phenylalanine), alkaloids (such as indoleacetic acid) and other compounds. Metabolomic analysis revealed that the dual function of phosphate-solubilizing and growth-promoting of Acinetobacter S1 depends on the reprogramming of central carbon metabolism (especially the pentose phosphate pathway, citric acid cycle and glycolysis / gluconeogenesis) to provide energy and carbon skeleton, and the activation of secondary metabolism (especially the biosynthesis of multiple amino acids) to supply plant growth precursors. In summary, it is feasible to use Acinetobacter S1 fermentation to produce a fermentation broth with phosphorus-solubilizing ability and rich in various plant probiotics using HDGS as a substrate.

Graphical Abstract