<p>Pumpkin seeds are recognized as a healthy snack and food ingredient that represents an important food industry sector, which generates considerable by-products; since once seeds are obtained (6–9% of the pumpkin fruit), the rest, mainly pulp and peels, is discarded as waste or underutilized as animal feed, despite being a rich source of nutrients and carotenoids. The present study reports on the valorization of pumpkin by-products for enriching canola oil via ultrasound-assisted extraction (UAE) of carotenoids. The influence of process variables (amplitude level, sonication time, and duty cycle) on the carotenoid extraction yield (CEY) was investigated, and the UAE conditions were optimized using response surface methodology (RSM) to maximize CEY. The effect of the UAE process on the physicochemical parameters of canola oil was evaluated. Additionally, the bioaccessibility and stability of carotenoids in the enriched canola oil were determined. Optimal UAE conditions were achieved at 69.7% amplitude, 17&#xa0;min sonication time, and 71.1% duty cycle. Under these conditions, 74.9% of the total carotenoid content (TCC) present in the pumpkin pulp was recovered.</p><p>Ultrasound application significantly (<i>p</i> &lt; 0.05) increased peroxide, anisidine, and total oxidation (TOTOX) values, negatively affecting canola oil quality. Carotenoid-enriched canola oil (CECO) represents an essential natural source of carotenoids (64.55 ± 2.85&#xa0;µg β-carotene equivalents/g oil), with high bioaccessibility (60.64 ± 0.27%) and retention (85%) during storage (4&#xa0;°C, 60&#xa0;days). CECO is a promising alternative to synthetic food colorants and a potential ingredient for functional foods, supplements, nutraceuticals, and cosmetics.</p> Graphical abstract

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Sustainable Recovery of Carotenoids from Pumpkin By-Products via Green Extraction Technologies

  • Rubén Portillo-López,
  • Gabriel I. Arguijo-Lozano,
  • Juliana Morales-Castro,
  • Luz A. Ochoa-Martínez,
  • J. Basilio Heredia,
  • María D. Muy-Rangel,
  • María I. Guerra-Rosas

摘要

Pumpkin seeds are recognized as a healthy snack and food ingredient that represents an important food industry sector, which generates considerable by-products; since once seeds are obtained (6–9% of the pumpkin fruit), the rest, mainly pulp and peels, is discarded as waste or underutilized as animal feed, despite being a rich source of nutrients and carotenoids. The present study reports on the valorization of pumpkin by-products for enriching canola oil via ultrasound-assisted extraction (UAE) of carotenoids. The influence of process variables (amplitude level, sonication time, and duty cycle) on the carotenoid extraction yield (CEY) was investigated, and the UAE conditions were optimized using response surface methodology (RSM) to maximize CEY. The effect of the UAE process on the physicochemical parameters of canola oil was evaluated. Additionally, the bioaccessibility and stability of carotenoids in the enriched canola oil were determined. Optimal UAE conditions were achieved at 69.7% amplitude, 17 min sonication time, and 71.1% duty cycle. Under these conditions, 74.9% of the total carotenoid content (TCC) present in the pumpkin pulp was recovered.

Ultrasound application significantly (p < 0.05) increased peroxide, anisidine, and total oxidation (TOTOX) values, negatively affecting canola oil quality. Carotenoid-enriched canola oil (CECO) represents an essential natural source of carotenoids (64.55 ± 2.85 µg β-carotene equivalents/g oil), with high bioaccessibility (60.64 ± 0.27%) and retention (85%) during storage (4 °C, 60 days). CECO is a promising alternative to synthetic food colorants and a potential ingredient for functional foods, supplements, nutraceuticals, and cosmetics.

Graphical abstract