Integration of Bagasse Pretreatment Black Liquor into Microbial Biopolymer Production – Process Modeling and Optimization
摘要
The release of black liquor (BL) generated from alkaline pretreatment of lignocellulosic biomass is an environmental burden. In this work, neutralized BL from sugarcane bagasse pretreatment was utilized in microbial production of polyhydroxyalkanoates and pullulan, employing either a constant total COD (CT-COD) or constant sucrose COD (CS-COD) experimental setup. More than 50% of the original COD could be utilized in CT-COD sets, where biomass and polymer production remain significantly higher than in BL-free control. Hyperparameter-optimized ANN models mapping COD utilization, biomass growth, and polymer production, developed using composite data, offered a reasonably good fit (R2: 0.936–0.988; R2CV: 0.878–0.978). The individual optimization of process responses suggests a maximum COD utilization of 11.79 g L−1 in Pullulan production, with an initial sucrose and BL COD of 21.62 g L−1 and 2.78 g L−1, respectively.
Graphical abstract