Scholarlink

<p>This study aims to explore the detoxification mechanisms of calcium peroxide (CaO₂) on viruses, pathogens, virulence factors (VFs), transport proteins (TPs), quorum sensing (QS), and total extracellular polymeric substances (TEPS) during sludge composting. The results indicate that, upon composting completion, the germination index (GI) of seeds in the treatment group (T) was 24.5% higher than in the control group (CK). The concentrations of toxic heavy metals As and Ni were reduced by 2.08&#xa0;mg/kg and 10.41&#xa0;mg/kg, respectively, compared to CK. Furthermore, the abundances of viruses such as <i>g__unclassified_f__Mimiviridae</i>, <i>g__Grisebachstrassevirus</i>, <i>g__Moturavirus</i> were significantly lower relative to CK. The addition of CaO₂ decreased the overall activity of pathogens, suppressed the functions and expression of VFs, and promoted the synthesis and expression of TPs, enhancing the activity of associated microorganisms and facilitating organic matter decomposition. Metagenomics and network analysis revealed that CaO₂ addition inhibited pathogen growth and altered metabolic pathways between pathogens, VFs, and TPs. Finally, correlation heatmap analysis confirmed that CaO₂, through a Fenton-like reaction, released reactive oxygen species (ROS) that suppressed QS capabilities, reduced TEPS content, enhanced the expression of TPs, decreased compost toxicity, and promoted humification. These findings suggest that under the influence of CaO₂, the toxicity of heavy metals, viruses, pathogens, and VFs in sludge composting is mitigated, with all showing significant suppression.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Calcium Peroxide Suppresses Pathogenicity and Reduces Toxicity During Sludge Composting by Inhibiting Quorum Sensing

  • 期刊论文

  • 发表日期：

    2025年12月5日

查看全文
  • Heng Lu,
  • Qunliang Li

摘要

This study aims to explore the detoxification mechanisms of calcium peroxide (CaO₂) on viruses, pathogens, virulence factors (VFs), transport proteins (TPs), quorum sensing (QS), and total extracellular polymeric substances (TEPS) during sludge composting. The results indicate that, upon composting completion, the germination index (GI) of seeds in the treatment group (T) was 24.5% higher than in the control group (CK). The concentrations of toxic heavy metals As and Ni were reduced by 2.08 mg/kg and 10.41 mg/kg, respectively, compared to CK. Furthermore, the abundances of viruses such as g__unclassified_f__Mimiviridae, g__Grisebachstrassevirus, g__Moturavirus were significantly lower relative to CK. The addition of CaO₂ decreased the overall activity of pathogens, suppressed the functions and expression of VFs, and promoted the synthesis and expression of TPs, enhancing the activity of associated microorganisms and facilitating organic matter decomposition. Metagenomics and network analysis revealed that CaO₂ addition inhibited pathogen growth and altered metabolic pathways between pathogens, VFs, and TPs. Finally, correlation heatmap analysis confirmed that CaO₂, through a Fenton-like reaction, released reactive oxygen species (ROS) that suppressed QS capabilities, reduced TEPS content, enhanced the expression of TPs, decreased compost toxicity, and promoted humification. These findings suggest that under the influence of CaO₂, the toxicity of heavy metals, viruses, pathogens, and VFs in sludge composting is mitigated, with all showing significant suppression.

Graphical abstract