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Purpose <p>The production of biomass and metabolites by lactic acid bacteria has driven research into alternative natural sources that enhance bacterial growth. Carob (<i>Ceratonia siliqua</i> L.), abundant in Algeria, presents technological and economic potential for this purpose. This study aims to optimize the composition of an innovative carob juice-based culture medium using response surface methodology.</p> Methods <p>A derived M17 and MRS culture medium enriched with carob juice was developed. A Plackett-Burman design identified significant variables, followed by a Central Composite Design (CCD) with 30 tests for four factors: carob juice, yeast extract, meat extract, and buffer (phosphate or sodium acetate). Optimal concentrations were determined using desirability profiles.</p> Results <p>Statistical analysis of the model revealed a significant influence of carob juice on the composition of the new culture media. For CJM1, the optimal conditions were, 14% carob juice, 4.5&#xa0;g/l yeast extract, 4.5&#xa0;g/l meat extract, and 300 mM phosphate buffer, achieving a 28% increase in cell density (from 3.337 to 4.609). For CJM2, 14% carob juice, 4&#xa0;g/l yeast extract, 8&#xa0;g/l meat extract, and 5&#xa0;g/l sodium acetate yielded results comparable to the MRS medium. Both models demonstrated robustness with a coefficient of determination R² of 0.95.</p> Conclusion <p>The results obtained, demonstrate clearly that carob juice is a key ingredient for cost-effective culture media, ensuring high yields for lactic acid bacteria growth. This resource offers a promising strategy to reduce production costs of culture media while maintaining efficiency.</p>

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Application of Plackett-Burman and Response Surface Methodology for the Optimization of Carob Juice-Based Culture Media for the Growth of Two Lactic Acid Bacteria Strains

  • 期刊论文

  • 发表日期：

    2025年11月21日

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  • Kaouter Bencherif,
  • Omar Hassaine

摘要

Purpose

The production of biomass and metabolites by lactic acid bacteria has driven research into alternative natural sources that enhance bacterial growth. Carob (Ceratonia siliqua L.), abundant in Algeria, presents technological and economic potential for this purpose. This study aims to optimize the composition of an innovative carob juice-based culture medium using response surface methodology.

Methods

A derived M17 and MRS culture medium enriched with carob juice was developed. A Plackett-Burman design identified significant variables, followed by a Central Composite Design (CCD) with 30 tests for four factors: carob juice, yeast extract, meat extract, and buffer (phosphate or sodium acetate). Optimal concentrations were determined using desirability profiles.

Results

Statistical analysis of the model revealed a significant influence of carob juice on the composition of the new culture media. For CJM1, the optimal conditions were, 14% carob juice, 4.5 g/l yeast extract, 4.5 g/l meat extract, and 300 mM phosphate buffer, achieving a 28% increase in cell density (from 3.337 to 4.609). For CJM2, 14% carob juice, 4 g/l yeast extract, 8 g/l meat extract, and 5 g/l sodium acetate yielded results comparable to the MRS medium. Both models demonstrated robustness with a coefficient of determination R² of 0.95.

Conclusion

The results obtained, demonstrate clearly that carob juice is a key ingredient for cost-effective culture media, ensuring high yields for lactic acid bacteria growth. This resource offers a promising strategy to reduce production costs of culture media while maintaining efficiency.