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<p>Cello-oligosaccharide (COS) has emerged as a sustainable and effective alternative to antibiotics in broiler chicken farming. The production process uses endoglucanase and exoglucanase with sugarcane bagasse as the substrate. This study explored two pretreatment methods—dilute acid and alkaline—both of which significantly increased the bagasse cellulose content to 75.6% and 71.1% w/w, respectively. Enzymatic hydrolysis using 10 U/g bagasse of endoglucanase yielded higher COS production from the acid-pretreated bagasse than from alkali-pretreated bagasse. Additionally, the introduction of 10 FPU/g bagasse of exoglucanase further broke down cellulose chains, enhancing COS production with notable prebiotic properties. Sequential enzyme addition yielded 92 ± 3&#xa0;mg COS/g bagasse, nearly double that of simultaneous addition (51 ± 10&#xa0;mg/g). In addition, the COS liquid demonstrated resilience to gastrointestinal conditions and effectively promoted the growth of beneficial probiotic strains, particularly <i>Bacillus subtilis</i> and <i>Lactobacillus</i> sp., surpassing the effectiveness of fructooligosaccharides (FOS) and glucose. This study demonstrates the potential of repurposing sugarcane bagasse for sustainable COS production, presenting a streamlined and scalable process that addresses waste management and the need for antibiotic alternatives in poultry farming.</p> Graphical Abstract <p></p>

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Sequential Enzyme Addition for the Enhanced Production of Cello-Oligosaccharides from Sugarcane Bagasse: A Sustainable Antibiotic Alternative To Broiler Farming

  • 期刊论文

  • 发表日期：

    2025年11月8日

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  • Nardrapee Karuna,
  • Patsamon Sangpundngam,
  • Hattaya Jinthaworn,
  • Wannee Tinthongkhob,
  • Pichet Sriboonyong,
  • Phimchanok Jaturapiree

摘要

Cello-oligosaccharide (COS) has emerged as a sustainable and effective alternative to antibiotics in broiler chicken farming. The production process uses endoglucanase and exoglucanase with sugarcane bagasse as the substrate. This study explored two pretreatment methods—dilute acid and alkaline—both of which significantly increased the bagasse cellulose content to 75.6% and 71.1% w/w, respectively. Enzymatic hydrolysis using 10 U/g bagasse of endoglucanase yielded higher COS production from the acid-pretreated bagasse than from alkali-pretreated bagasse. Additionally, the introduction of 10 FPU/g bagasse of exoglucanase further broke down cellulose chains, enhancing COS production with notable prebiotic properties. Sequential enzyme addition yielded 92 ± 3 mg COS/g bagasse, nearly double that of simultaneous addition (51 ± 10 mg/g). In addition, the COS liquid demonstrated resilience to gastrointestinal conditions and effectively promoted the growth of beneficial probiotic strains, particularly Bacillus subtilis and Lactobacillus sp., surpassing the effectiveness of fructooligosaccharides (FOS) and glucose. This study demonstrates the potential of repurposing sugarcane bagasse for sustainable COS production, presenting a streamlined and scalable process that addresses waste management and the need for antibiotic alternatives in poultry farming.

Graphical Abstract