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<p>This study aims to synthesize silica nanoparticles (SiO<sub>2</sub>-NPs) using <i>Tridax procumbens</i> leaves. Previous studies have demonstrated the effectiveness of the leaf juice of <i>Tridax procumbens</i> in treating and healing fresh wounds. SiO<sub>2</sub>-NPs have been found to enhance topical wound healing by promoting the migration of skin fibroblasts. Therefore, the current research focused on the green synthesis of SiO<sub>2</sub>-NPs from <i>Tridax procumbens</i> leaves extract and evaluating their scratch wound healing activities. The synthesized SiO<sub>2</sub>-NPs were characterized using various techniques like XRD, UV-visible spectrophotometry, FTIR, FESEM, EDX, Zeta potential and TG analysis. The characterization analysis confirmed that the particles were spherical, containing silicon (Si) and oxygen (O), exhibiting an average size of 44&#xa0;nm, and experiencing a mass loss of 48.9%. The antioxidant activity of the synthesized SiO<sub>2</sub>-NPs was evaluated through the DPPH assay, revealing an IC50 value of 48.8&#xa0;µg/mL. This indicates their capability to diminish free radical generation and alleviate oxidative stress. Moreover, the study aimed to explore the efficacy of these nanoparticles in wound healing activity. The in vitro scratch assay was employed to assess wound closure of L929 fibroblast cell lines. The results revealed that the synthesized SiO<sub>2</sub>-NPs showed higher wound healing activity (74%) in L929 fibroblast cell lines than the control at 24&#xa0;h, indicating the ability of the synthesized nanoparticles in enhancing wound closure processes. Overall, this study analyses the potential of plant-mediated synthesis of SiO<sub>2</sub>-NPs for biomedical applications, particularly in wound healing therapies.</p>

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Green Synthesis and Characterization of SiO2 Nanoparticles Using Tridax Procumbens Leaf Extract and Enhancing the Invitro Wound Healing Activity in L929 Fibroblast Cell Lines

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月24日

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  • Vanathi Palanimuthu,
  • Nishanthi Rajendran,
  • Rajiv Periakaruppan,
  • Valentin Romanovski,
  • Priyanka Govindaraju,
  • Mani Natarajan

摘要

This study aims to synthesize silica nanoparticles (SiO2-NPs) using Tridax procumbens leaves. Previous studies have demonstrated the effectiveness of the leaf juice of Tridax procumbens in treating and healing fresh wounds. SiO2-NPs have been found to enhance topical wound healing by promoting the migration of skin fibroblasts. Therefore, the current research focused on the green synthesis of SiO2-NPs from Tridax procumbens leaves extract and evaluating their scratch wound healing activities. The synthesized SiO2-NPs were characterized using various techniques like XRD, UV-visible spectrophotometry, FTIR, FESEM, EDX, Zeta potential and TG analysis. The characterization analysis confirmed that the particles were spherical, containing silicon (Si) and oxygen (O), exhibiting an average size of 44 nm, and experiencing a mass loss of 48.9%. The antioxidant activity of the synthesized SiO2-NPs was evaluated through the DPPH assay, revealing an IC50 value of 48.8 µg/mL. This indicates their capability to diminish free radical generation and alleviate oxidative stress. Moreover, the study aimed to explore the efficacy of these nanoparticles in wound healing activity. The in vitro scratch assay was employed to assess wound closure of L929 fibroblast cell lines. The results revealed that the synthesized SiO2-NPs showed higher wound healing activity (74%) in L929 fibroblast cell lines than the control at 24 h, indicating the ability of the synthesized nanoparticles in enhancing wound closure processes. Overall, this study analyses the potential of plant-mediated synthesis of SiO2-NPs for biomedical applications, particularly in wound healing therapies.