Scholarlink

<p>Poultry feather waste poses a significant environmental challenge due to the recalcitrant nature of keratin. This study reports the isolation and characterization of <i>Bacillus amyloliquefaciens</i> NS56, a food-grade soil bacterium demonstrating robust keratinolytic activity under minimal-input fermentation conditions. Identified through 16&#xa0;S rRNA sequencing and phylogenetic analysis, NS56 achieved complete feather degradation within seven days, producing 142 U/mL of keratinase—rising to 162 U/mL with FeSO₄ supplementation. The enzyme exhibited optimal activity at pH 7.0 and 50&#xa0;°C, maintaining stability across neutral to mildly alkaline conditions. Fe²⁺ significantly enhanced activity (up to 187.5%) whereas Zn²⁺ strongly inhibited it; Dithiothreitol and fresh medium supplementation further accelerated degradation kinetics. SDS-PAGE confirmed broad proteolytic activity against casein, gelatin and bovine serum albumin, indicating potential applications beyond keratin substrates. Importantly, the process required no genetic modification or enzyme purification, underscoring NS56’s native suitability as a direct whole-cell biocatalyst. This low-input approach streamlines processing while preserving high keratinolytic efficiency. Its food-grade classification supports potential co-cultivation with lactic acid bacteria, paving the way for eco-friendly bioprocess development. Collectively, these findings position NS56 as a scalable and sustainable solution for feather waste valorization within circular bioeconomy frameworks.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Food-Grade Bacillus amyloliquefaciens NS56 as a Whole Cell Biocatalyst for Sustainable Feather Waste Valorization

  • 期刊论文

  • 发表日期：

    2025年10月21日

查看全文
  • Nazar Hussain,
  • Muhammad Tariq,
  • Mingchen Yan,
  • Tahir Naqqash,
  • Zeenat Fatima,
  • Muhammad Naeem

摘要

Poultry feather waste poses a significant environmental challenge due to the recalcitrant nature of keratin. This study reports the isolation and characterization of Bacillus amyloliquefaciens NS56, a food-grade soil bacterium demonstrating robust keratinolytic activity under minimal-input fermentation conditions. Identified through 16 S rRNA sequencing and phylogenetic analysis, NS56 achieved complete feather degradation within seven days, producing 142 U/mL of keratinase—rising to 162 U/mL with FeSO₄ supplementation. The enzyme exhibited optimal activity at pH 7.0 and 50 °C, maintaining stability across neutral to mildly alkaline conditions. Fe²⁺ significantly enhanced activity (up to 187.5%) whereas Zn²⁺ strongly inhibited it; Dithiothreitol and fresh medium supplementation further accelerated degradation kinetics. SDS-PAGE confirmed broad proteolytic activity against casein, gelatin and bovine serum albumin, indicating potential applications beyond keratin substrates. Importantly, the process required no genetic modification or enzyme purification, underscoring NS56’s native suitability as a direct whole-cell biocatalyst. This low-input approach streamlines processing while preserving high keratinolytic efficiency. Its food-grade classification supports potential co-cultivation with lactic acid bacteria, paving the way for eco-friendly bioprocess development. Collectively, these findings position NS56 as a scalable and sustainable solution for feather waste valorization within circular bioeconomy frameworks.