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<p>The exuberance of microbial consortia includes the complementary metabolism that assists in breaking down lignocellulosic biomass. Composting organic waste is considered an efficient method for carbon recycling, with syntrophic microbial populations working together to decompose the lignin and polysaccharide components of plant biomass. Therefore, this study aimed to develop a microbial consortium for the effective degradation of lignocellulosic biomass. The microbes were isolated from various environmental samples such as decayed teak wood, termite guts, elephant dung, and tropical forest soil. The isolated microbes were tested for production of endoglucanase, exoglucanase, β-glucosidase, xylanase, laccase, lignin peroxidase, and manganese peroxidase. The results showed that three bacteria, one actinobacterium, and three fungal isolates were highly proficient in producing lignin-degrading enzymes. Upon testing their compatibility and identifying through phylogenetic analysis, it was found that the bacterial culture was <i>Stenotrophomonas maltophilia</i>, the actinobacterial culture was <i>Cellulomonas flavigena</i>, and the fungal isolates were <i>Trichoderma yunnanense</i>, <i>Penicillium citrinum</i>, and <i>Phanerodontia chrysosporium</i>. Consequently, a consortium was developed using these microbes, and its effectiveness in degrading lignocellulosic materials were tested in vitro using rice straw and maize stalk. The results showed the consortium achieved the lowest organic carbon content of 32.98% and 35.09%, highest total nitrogen content of 1.05% and 0.95%, and lowest C/N ratio of 31.40:1 and 36.93:1 in rice straw and maize stalk, respectively in 20 days incubation period. Thus, the consortium developed in this study has the potential to accelerate the composting of lignocellulosic biomass.</p>

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Elucidating the Effectiveness of Microbial Isolates from Different Ecological Niches and Their Associated Enzymes in Breaking down Lignocellulosic Biomass Through In - Vitro Experiments

  • 期刊论文

  • 发表日期：

    2025年9月18日

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  • Periasamy Kalaiselvi,
  • Balakrishnan Mariammal Porkavi,
  • Selvaraj Paul Sebastian,
  • Venkatakrishnan Sivaraj Saravanan,
  • Veeraswamy Davamani,
  • Ettiyagounder Parameswari,
  • Ramesh Poornima,
  • Tamilselvan Ilakiya

摘要

The exuberance of microbial consortia includes the complementary metabolism that assists in breaking down lignocellulosic biomass. Composting organic waste is considered an efficient method for carbon recycling, with syntrophic microbial populations working together to decompose the lignin and polysaccharide components of plant biomass. Therefore, this study aimed to develop a microbial consortium for the effective degradation of lignocellulosic biomass. The microbes were isolated from various environmental samples such as decayed teak wood, termite guts, elephant dung, and tropical forest soil. The isolated microbes were tested for production of endoglucanase, exoglucanase, β-glucosidase, xylanase, laccase, lignin peroxidase, and manganese peroxidase. The results showed that three bacteria, one actinobacterium, and three fungal isolates were highly proficient in producing lignin-degrading enzymes. Upon testing their compatibility and identifying through phylogenetic analysis, it was found that the bacterial culture was Stenotrophomonas maltophilia, the actinobacterial culture was Cellulomonas flavigena, and the fungal isolates were Trichoderma yunnanense, Penicillium citrinum, and Phanerodontia chrysosporium. Consequently, a consortium was developed using these microbes, and its effectiveness in degrading lignocellulosic materials were tested in vitro using rice straw and maize stalk. The results showed the consortium achieved the lowest organic carbon content of 32.98% and 35.09%, highest total nitrogen content of 1.05% and 0.95%, and lowest C/N ratio of 31.40:1 and 36.93:1 in rice straw and maize stalk, respectively in 20 days incubation period. Thus, the consortium developed in this study has the potential to accelerate the composting of lignocellulosic biomass.