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<p>β-Glucosidases (βG) play a crucial role in the hydrolysis of cellulose to glucose, a key step in the production of high-value bioproducts. However, the high cost of these enzymes limits their broader industrial application. Solid-state fermentation (SSF) using lignocellulosic byproducts as culture medium offers a cost-effective alternative for βG production. This study aimed to maximize the production of crude extract with βG activity (CE-βG) by <i>Ganoderma lucidum</i> through SSF using Oil Palm Mesocarp Fiber (OPMF), a cellulose-rich waste from crude palm oil extraction. The effects of pH and temperature on enzyme activity were evaluated, along with its application in corn husk saccharification. The CE-βG exhibited optimal activity at 50&#xa0;°C, retaining 50% of its initial activity after 3&#xa0;h at this temperature. It demonstrated an acidophilic nature, with enhanced activity at pH 5. Direct application of the enzymatic extract to corn husk saccharification resulted in a significant release of reducing sugars (147.47&#xa0;mg g⁻¹), which may minimize the requirement for further purification, supplementation, or pretreatment. The CE-βG proved efficient in sugar production from lignocellulosic biomass, with potential for further optimization in saccharification processes or simultaneous fermentation to produce alcohols, biofuels, organic acids, and biosurfactants. CE-βG not only stands out for its exceptional stability and acidophilic nature but also represents a potential for scaling approach to sustainable bioprocessing, unlocking new possibilities for the efficient valorization of agri-food byproducts into industrial compounds. CE-βG holds the potential to drive industrial-scale applications in biofuels and bioproducts, with reduced production costs and a lower environmental impact.</p>

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Transforming Palm Oil Waste: High-Performance Crude Extract for Efficient Saccharification of Corn Husk by-products

  • 期刊论文

  • 发表日期：

    2025年8月26日

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  • Eliézer Luz do Espirito Santo,
  • Kendria Santos Cezar,
  • Igor Carvalho Fontes Sampaio,
  • Isabela Viana Lopes de Moura,
  • Fabiane Neves Silva,
  • Sabryna Couto Araujo,
  • Muhammad Irfan,
  • Julieta Rangel de Oliveira,
  • Marcelo Franco

摘要

β-Glucosidases (βG) play a crucial role in the hydrolysis of cellulose to glucose, a key step in the production of high-value bioproducts. However, the high cost of these enzymes limits their broader industrial application. Solid-state fermentation (SSF) using lignocellulosic byproducts as culture medium offers a cost-effective alternative for βG production. This study aimed to maximize the production of crude extract with βG activity (CE-βG) by Ganoderma lucidum through SSF using Oil Palm Mesocarp Fiber (OPMF), a cellulose-rich waste from crude palm oil extraction. The effects of pH and temperature on enzyme activity were evaluated, along with its application in corn husk saccharification. The CE-βG exhibited optimal activity at 50 °C, retaining 50% of its initial activity after 3 h at this temperature. It demonstrated an acidophilic nature, with enhanced activity at pH 5. Direct application of the enzymatic extract to corn husk saccharification resulted in a significant release of reducing sugars (147.47 mg g⁻¹), which may minimize the requirement for further purification, supplementation, or pretreatment. The CE-βG proved efficient in sugar production from lignocellulosic biomass, with potential for further optimization in saccharification processes or simultaneous fermentation to produce alcohols, biofuels, organic acids, and biosurfactants. CE-βG not only stands out for its exceptional stability and acidophilic nature but also represents a potential for scaling approach to sustainable bioprocessing, unlocking new possibilities for the efficient valorization of agri-food byproducts into industrial compounds. CE-βG holds the potential to drive industrial-scale applications in biofuels and bioproducts, with reduced production costs and a lower environmental impact.