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Purpose <p>Poly(butylene succinate) (PBS)-degrading enzyme produced by a newly isolated thermophilic actinobacterium <i>Microbispora rosea</i> BS2-4 was investigated. The crude enzyme was characterized and applied to degrade PBS film to address environmental waste pollution.</p> Methods <p>The medium and physical parameters affecting PBS-degrading enzyme production were investigated including pH, temperature, nitrogen sources, and metal ions. Factors influencing crude enzyme activity were examined, and the degradation of PBS film was tested at a concentration of 1,000&#xa0;mg/L. The enzymes involved in PBS degradation by the BS2-4 strain were also evaluated.</p> Results <p>The optimal conditions for enzyme production were pH 9.0 and 50&#xa0;°C. The addition of silk fibrous waste (SFW), a low-cost agricultural byproduct typically discarded in silk textile processes at 4.0&#xa0;g/L along with MgSO₄·7&#xa0;H₂O (0.4&#xa0;g/L) to the minimal medium enhanced enzyme production to 0.45 ± 0.01 U/mL, resulting in a 1.22-fold improvement and a 3.71-fold reduction in cost compared to the non-optimized medium. The crude enzyme degraded PBS film after 72&#xa0;h of incubation at 60&#xa0;°C, as confirmed by significant changes in its physical and chemical structure.</p> Conclusions <p>This study identified a potent strain producing PBS-degrading enzymes, which showed promise as an alternative solution to ameliorate future PBS waste accumulation.</p>

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Sustainable Utilization of Silk Fibrous Waste (SFW) for Poly(butylene succinate) (PBS)-Degrading Enzyme Production by Microbispora rosea BS2-4 and its Degradative Capability on PBS Film Polymer

  • 期刊论文

  • 发表日期：

    2025年8月5日

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  • Ausawadee Phonlamai,
  • Voranuch Thongpool,
  • Chatsuda Sakdapetsiri,
  • Vichien Kitpreechavanich,
  • Thanasak Lomthong

摘要

Purpose

Poly(butylene succinate) (PBS)-degrading enzyme produced by a newly isolated thermophilic actinobacterium Microbispora rosea BS2-4 was investigated. The crude enzyme was characterized and applied to degrade PBS film to address environmental waste pollution.

Methods

The medium and physical parameters affecting PBS-degrading enzyme production were investigated including pH, temperature, nitrogen sources, and metal ions. Factors influencing crude enzyme activity were examined, and the degradation of PBS film was tested at a concentration of 1,000 mg/L. The enzymes involved in PBS degradation by the BS2-4 strain were also evaluated.

Results

The optimal conditions for enzyme production were pH 9.0 and 50 °C. The addition of silk fibrous waste (SFW), a low-cost agricultural byproduct typically discarded in silk textile processes at 4.0 g/L along with MgSO₄·7 H₂O (0.4 g/L) to the minimal medium enhanced enzyme production to 0.45 ± 0.01 U/mL, resulting in a 1.22-fold improvement and a 3.71-fold reduction in cost compared to the non-optimized medium. The crude enzyme degraded PBS film after 72 h of incubation at 60 °C, as confirmed by significant changes in its physical and chemical structure.

Conclusions

This study identified a potent strain producing PBS-degrading enzymes, which showed promise as an alternative solution to ameliorate future PBS waste accumulation.