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<p>Globally, an enormous volume of agri-industrial residues is generated each year, which can lead to environmental issues. The work aimed to screen various agro-industrial by-products and optimize the physicochemical parameters of solid-state fermentation using response surface design for the applicability of crude lipase as a supplement in the detergent industry. Four agri-residues (cotton seed oil cake, wheat bran, soybean meal, rice husk) were screened to obtain the highest lipase activity, 2.81 ± 0.25 U/gds from <i>Aspergillus japonicus</i> MTCC 1975 using soybean meal. The optimization studies were conducted for lipase production using the best substrate. Initially, one factor at a time classical method was used to identify the variables which have a crucial role in lipase production. The significant factors were studied by central composite design (CCD) to achieve the maximum lipase production of 15.29 U/gds (with 5.4-fold increase) at 2% w/w of CSL (corn steep liquor), 1% v/w of Tween 20 and 65% w/w moisture content compared to un-optimized conditions. Further, crude lipase was examined for stain removal efficiency with commercial detergent. This study can help the exploration of locally available agri-residues for the production of extracellular fungal lipase that can be utilized as an additive to commercial detergents for improving stain removal ability.</p>

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Optimization of Fungal Lipase Production Through Valorisation of Agri-Residue Soybean Meal Substrate in Solid-State Fermentation for Potential Application as Detergent Additive

  • 期刊论文

  • 发表日期：

    2025年7月25日

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  • Saheli Sabnam,
  • Bhim Singh,
  • Asim Kumar Jana,
  • Mithu Maiti Jana

摘要

Globally, an enormous volume of agri-industrial residues is generated each year, which can lead to environmental issues. The work aimed to screen various agro-industrial by-products and optimize the physicochemical parameters of solid-state fermentation using response surface design for the applicability of crude lipase as a supplement in the detergent industry. Four agri-residues (cotton seed oil cake, wheat bran, soybean meal, rice husk) were screened to obtain the highest lipase activity, 2.81 ± 0.25 U/gds from Aspergillus japonicus MTCC 1975 using soybean meal. The optimization studies were conducted for lipase production using the best substrate. Initially, one factor at a time classical method was used to identify the variables which have a crucial role in lipase production. The significant factors were studied by central composite design (CCD) to achieve the maximum lipase production of 15.29 U/gds (with 5.4-fold increase) at 2% w/w of CSL (corn steep liquor), 1% v/w of Tween 20 and 65% w/w moisture content compared to un-optimized conditions. Further, crude lipase was examined for stain removal efficiency with commercial detergent. This study can help the exploration of locally available agri-residues for the production of extracellular fungal lipase that can be utilized as an additive to commercial detergents for improving stain removal ability.