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Purpose <p>This study aims to evaluate the potential of brown seaweed <i>Sargassum</i> sp. as a sole carbon source for cultivating marine endophytic fungus <i>Aspergillus flavus</i> KTR49 and to characterize its exopolysaccharides.</p> Methods <p>Marine endophytic <i>Aspergillus flavus</i> KTR49 was cultivated using a liquid medium with <i>Sargassum</i> sp. and synthetic glucose as the sole carbon source. A control without glucose and <i>Sargassum</i> sp. was included to assess growth under carbon-limited conditions. After 7 days of incubation, extracellular precipitates were collected and analyzed. Exopolysaccharides were then characterized to determine their functional groups (FTIR), zeta potential, molecular weight (GPC), biocompatibility (MTT cytotoxicity assay), and thermal stability (TGA).</p> Results <p>The highest reducing sugar concentration was observed on day 5 in the <i>Sargassum</i> sp. medium before declining. The exopolysaccharide (EPS) yield in the <i>Sargassum sp.</i> medium reached 3183.33 ± 329.08&#xa0;mg/L, with a molecular weight of 302,55&#xa0;KDa at the highest peaks. Zeta potential analysis showed a higher negative charge (-25.21 ± 0.16 mV), indicating stability, while thermogravimetric analysis confirmed good thermal resistance. The MTT assay revealed that EPS maintained high cell viability (&gt; 77%), confirming its biocompatibility.</p> Conclusion <p>These findings suggest that <i>Sargassum</i> sp. is an effective and sustainable carbon source for fungus cultivation, supporting gradual sugar release, enhanced EPS production, and favorable physicochemical properties. This study highlights <i>Sargassum</i> sp. as a viable alternative to synthetic carbon sources for marine biotechnology applications.</p>

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Valorization of the Brown Seaweed Sargassum sp. As a Sole Carbon Source for Marine Endophytic Fungus Aspergillus Flavus KTR49 Cultivation

  • 期刊论文

  • 发表日期：

    2025年7月24日

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  • Safrina Dyah Hardiningtyas,
  • Ghea Nur Haliza,
  • Kustiariyah Tarman,
  • Uju

摘要

Purpose

This study aims to evaluate the potential of brown seaweed Sargassum sp. as a sole carbon source for cultivating marine endophytic fungus Aspergillus flavus KTR49 and to characterize its exopolysaccharides.

Methods

Marine endophytic Aspergillus flavus KTR49 was cultivated using a liquid medium with Sargassum sp. and synthetic glucose as the sole carbon source. A control without glucose and Sargassum sp. was included to assess growth under carbon-limited conditions. After 7 days of incubation, extracellular precipitates were collected and analyzed. Exopolysaccharides were then characterized to determine their functional groups (FTIR), zeta potential, molecular weight (GPC), biocompatibility (MTT cytotoxicity assay), and thermal stability (TGA).

Results

The highest reducing sugar concentration was observed on day 5 in the Sargassum sp. medium before declining. The exopolysaccharide (EPS) yield in the Sargassum sp. medium reached 3183.33 ± 329.08 mg/L, with a molecular weight of 302,55 KDa at the highest peaks. Zeta potential analysis showed a higher negative charge (-25.21 ± 0.16 mV), indicating stability, while thermogravimetric analysis confirmed good thermal resistance. The MTT assay revealed that EPS maintained high cell viability (> 77%), confirming its biocompatibility.

Conclusion

These findings suggest that Sargassum sp. is an effective and sustainable carbon source for fungus cultivation, supporting gradual sugar release, enhanced EPS production, and favorable physicochemical properties. This study highlights Sargassum sp. as a viable alternative to synthetic carbon sources for marine biotechnology applications.