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Purpose <p>Converting lignocellulosic residues, like sugarcane bagasse, into single-cell proteins (SCPs) offers a sustainable protein production method to aid in ensuring global food security.</p> Methods <p>This study evaluated steam-explosion and enzymatic hydrolysis as pretreatment for sugarcane bagasse feedstock, with a subsequent investigation of SCP production from the pretreated sugarcane bagasse, individually employing six ‘generally-regarded-as-safe’ microorganisms: <i>Bacillus subtilis</i>,<i> Lactobacillus delbrueckii</i>,<i> Streptococcus thermophilus</i>,<i> Fusarium venenatum</i>,<i> Pleurotus ostreatus</i> and <i>Saccharomyces cerevisiae</i>. Microbial biomass growth and protein production were investigated under both batch as well as fed-batch fermentation conditions. Furthermore, an integrated SCP biorefinery was designed and simulated in Aspen Plus<sup>®</sup>, with a subsequent techno-economic analysis to determine the profitability of such a SCP biorefinery.</p> Results <p><i>S. cerevisiae</i> achieved the highest biomass concentration (20.37 ± 11.81&#xa0;g/L) in batch culture, while <i>S. thermophilus</i> also exhibited some tolerance to inhibitors produced during steam explosion (1.28 ± 0.17&#xa0;g/L). Pulsed fed-batch fermentations enhanced biomass growth, yielding biomass concentrations of 52.65 ± 0.80&#xa0;g/L and 6.57 ± 0.09&#xa0;g/L, and SCP concentrations of 24.71 ± 1.44&#xa0;g/L and 4.34 ± 0.10&#xa0;g/L, for <i>S. cerevisiae</i> and <i>S. thermophilus</i>, respectively. The cost of the <i>S. cerevisiae</i> bulk product (1.91 US$/kg) was considerably lower than that of <i>S. thermophilus</i> (15.51 US$/kg), further supporting its suitability for SCP co-production with bio-ethanol.</p> Conclusion <p>The production of SCP from steam-exploded and enzymatically hydrolyzed sugarcane bagasse was found to be profitable, with <i>S. cerevisiae</i> being the preferred ‘generally-regarded-as-safe’ microorganism for this process.</p>

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Bioconversion of Steam-Exploded Sugarcane Bagasse to Microbial Protein: An Experimental Investigation and Techno-Economic Analysis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月5日

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  • Sasha D. Kasperski,
  • Catharine E. Bosman,
  • Abdul M. Petersen,
  • Johann F. Görgens,
  • Eugéne van Rensburg

摘要

Purpose

Converting lignocellulosic residues, like sugarcane bagasse, into single-cell proteins (SCPs) offers a sustainable protein production method to aid in ensuring global food security.

Methods

This study evaluated steam-explosion and enzymatic hydrolysis as pretreatment for sugarcane bagasse feedstock, with a subsequent investigation of SCP production from the pretreated sugarcane bagasse, individually employing six ‘generally-regarded-as-safe’ microorganisms: Bacillus subtilis, Lactobacillus delbrueckii, Streptococcus thermophilus, Fusarium venenatum, Pleurotus ostreatus and Saccharomyces cerevisiae. Microbial biomass growth and protein production were investigated under both batch as well as fed-batch fermentation conditions. Furthermore, an integrated SCP biorefinery was designed and simulated in Aspen Plus®, with a subsequent techno-economic analysis to determine the profitability of such a SCP biorefinery.

Results

S. cerevisiae achieved the highest biomass concentration (20.37 ± 11.81 g/L) in batch culture, while S. thermophilus also exhibited some tolerance to inhibitors produced during steam explosion (1.28 ± 0.17 g/L). Pulsed fed-batch fermentations enhanced biomass growth, yielding biomass concentrations of 52.65 ± 0.80 g/L and 6.57 ± 0.09 g/L, and SCP concentrations of 24.71 ± 1.44 g/L and 4.34 ± 0.10 g/L, for S. cerevisiae and S. thermophilus, respectively. The cost of the S. cerevisiae bulk product (1.91 US$/kg) was considerably lower than that of S. thermophilus (15.51 US$/kg), further supporting its suitability for SCP co-production with bio-ethanol.

Conclusion

The production of SCP from steam-exploded and enzymatically hydrolyzed sugarcane bagasse was found to be profitable, with S. cerevisiae being the preferred ‘generally-regarded-as-safe’ microorganism for this process.