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<p>The plant-mediated synthesis of metallic nanoparticles is experiencing growing commercial demand due to its extensive applicability across diverse fields, including electronics, catalysis, chemistry, energy, cosmetics, and medicine. In the current investigation, the aqueous extract of bitter apple seeds (BAE) was prepared, characterized, and its antioxidant activity assessed. The extract’s ability to scavenge DPPH radicals increased from 28 to 79% when the concentration was raised from 25 to 500&#xa0;µg/mL. Also, Ag-NPs were synthesized with bitter apple (Ag-NPs) and subsequently characterized by UV-VIS absorbance, particle size, Polydispersity index (PdI), Zeta potential (ZP), and transmission electron microscopy (TEM). The phytochemicals present in bitter apple extract were used both as a reducing and a stabilizing agent for the synthesis of Ag-NPs. We measured the antibacterial activity against <i>Staphylococcus au</i>r<i>eus</i> and <i>Pseudomonas aeruginosa</i>. Also, the anticancer activity of BA-Ag-NPs against A549 and HCT116 cancer cells was estimated. Phytochemical analysis of BAE has shown the presence of phenolic compounds (7.11&#xa0;mg GAE/g extract). Gallic acid, syringic acid, pyrocatechol, ellagic acid, vanillin, naringenin, and cinnamic acid are the key seven components. The Ag-NPs were successfully prepared for green synthesized by the BAE. The particle size of the produced nanoparticles was measured using the Zeta Sizer equipment. The size formed Ag-NPs was 85 ± 5&#xa0;nm and the absorption spectrum of the Ag-NPs using UV-vis was 400&#xa0;nm. Results of the antibacterial evaluation indicated that Ag-NPs had highly antibacterial activity against <i>Staphylococcus au</i>r<i>eus</i> compared to <i>Pseudomonas aeruginosa</i>. The minimum inhibitory concentration (MIC) for <i>Staphylococcus au</i>r<i>eus</i> and <i>Pseudomonas aeruginosa</i> were recorded at 12 and 25&#xa0;µg/mL, respectively. By analyzing TEM images, different indicators of cell wall and membrane deformation in treated cells were observed. The possible bactericidal processes of green-Ag-NPs include the attachment and penetration of nanoparticles into the bacterial cell wall, disruption of respiration, DNA damage due to reactive oxygen species generation, and apoptosis. The Ag-NPs exhibit concentration-dependent inhibition of A549 and HCT116 cell proliferation. IC<sub>50</sub> for treated cells (A549 and HCT116) was recorded at 89, and 108&#xa0;µg/mL, respectively. The findings suggest that Ag-NPs produced with BAE and a green method could serve as a substitute antibacterial and anticancer agent.</p> Graphical Abstract <p></p>

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Biosynthesis of Silver Nanoparticles Using Bitter Apple Seed Extract: Anticancer and Antibacterial Activities

  • 期刊论文

  • 发表日期：

    2025年5月28日

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  • Hossam S. El-Beltagi,
  • Ali Osman,
  • Adel A. Rezk,
  • Wafaa Eid,
  • Wael F. Shehata,
  • Hend A. Elakkad,
  • Ahlam Saleh Alhajri,
  • Ragab A. El-Masry,
  • Abdelrahman R. Ahmed,
  • Ahmed A. El-Sayed,
  • Ahmed Mahmoud Ismail,
  • Mahmoud M. El-Saber

摘要

The plant-mediated synthesis of metallic nanoparticles is experiencing growing commercial demand due to its extensive applicability across diverse fields, including electronics, catalysis, chemistry, energy, cosmetics, and medicine. In the current investigation, the aqueous extract of bitter apple seeds (BAE) was prepared, characterized, and its antioxidant activity assessed. The extract’s ability to scavenge DPPH radicals increased from 28 to 79% when the concentration was raised from 25 to 500 µg/mL. Also, Ag-NPs were synthesized with bitter apple (Ag-NPs) and subsequently characterized by UV-VIS absorbance, particle size, Polydispersity index (PdI), Zeta potential (ZP), and transmission electron microscopy (TEM). The phytochemicals present in bitter apple extract were used both as a reducing and a stabilizing agent for the synthesis of Ag-NPs. We measured the antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. Also, the anticancer activity of BA-Ag-NPs against A549 and HCT116 cancer cells was estimated. Phytochemical analysis of BAE has shown the presence of phenolic compounds (7.11 mg GAE/g extract). Gallic acid, syringic acid, pyrocatechol, ellagic acid, vanillin, naringenin, and cinnamic acid are the key seven components. The Ag-NPs were successfully prepared for green synthesized by the BAE. The particle size of the produced nanoparticles was measured using the Zeta Sizer equipment. The size formed Ag-NPs was 85 ± 5 nm and the absorption spectrum of the Ag-NPs using UV-vis was 400 nm. Results of the antibacterial evaluation indicated that Ag-NPs had highly antibacterial activity against Staphylococcus aureus compared to Pseudomonas aeruginosa. The minimum inhibitory concentration (MIC) for Staphylococcus aureus and Pseudomonas aeruginosa were recorded at 12 and 25 µg/mL, respectively. By analyzing TEM images, different indicators of cell wall and membrane deformation in treated cells were observed. The possible bactericidal processes of green-Ag-NPs include the attachment and penetration of nanoparticles into the bacterial cell wall, disruption of respiration, DNA damage due to reactive oxygen species generation, and apoptosis. The Ag-NPs exhibit concentration-dependent inhibition of A549 and HCT116 cell proliferation. IC50 for treated cells (A549 and HCT116) was recorded at 89, and 108 µg/mL, respectively. The findings suggest that Ag-NPs produced with BAE and a green method could serve as a substitute antibacterial and anticancer agent.

Graphical Abstract