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<p>This study focuses on the extraction, purification and characterization of R-Phycoerythrin (R-PE) from <i>Hypnea musciformis</i>, a red seaweed species known for its high phycobiliprotein content. R-PE was extracted using a precipitation method with 55% ammonium sulfate, followed by purification through Q-Sepharose column chromatography, resulting in a maximum purity index 4.9. Spectral analyses revealed a fluorescence emission peak at 578&#xa0;nm and absorption peaks at 498&#xa0;nm, 536&#xa0;nm, and 566&#xa0;nm, characteristic of R-PE. Native- PAGE showed a uniform protein band at 240 KDa, confirming structural integrity post-purification. SDS-PAGE and 2D-Gel electrophoresis identified subunits (α, β, γ) and their molecular masses. These findings highlight R-PE’s robust purity and structural uniformity from <i>Hypnea musciformis</i>, making it suitable for potential applications in biotechnological and biomedical fields.</p>

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Purification and Analysis of R-Phycoerythrin from Hypnea musiformis: Insights into Structural Integrity and Purity

  • 期刊论文

  • 发表日期：

    2025年5月15日

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  • Gurusamy Subha Devi,
  • Madhavan Sathiyabama,
  • Namasivayam Senthilkumar,
  • Jeyaraj Ezekiel Raj,
  • Srinivasan Venkatesh,
  • Pitchai Murugan,
  • Malairaj Sathuvan

摘要

This study focuses on the extraction, purification and characterization of R-Phycoerythrin (R-PE) from Hypnea musciformis, a red seaweed species known for its high phycobiliprotein content. R-PE was extracted using a precipitation method with 55% ammonium sulfate, followed by purification through Q-Sepharose column chromatography, resulting in a maximum purity index 4.9. Spectral analyses revealed a fluorescence emission peak at 578 nm and absorption peaks at 498 nm, 536 nm, and 566 nm, characteristic of R-PE. Native- PAGE showed a uniform protein band at 240 KDa, confirming structural integrity post-purification. SDS-PAGE and 2D-Gel electrophoresis identified subunits (α, β, γ) and their molecular masses. These findings highlight R-PE’s robust purity and structural uniformity from Hypnea musciformis, making it suitable for potential applications in biotechnological and biomedical fields.