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<p>Bioplastics are the polymers synthesized from either microorganisms or they can be manufactured using low cost agricultural biomass. They are gaining considerable attention nowadays due to their biodegradability and provision of utilizing waste material. Bacteria are versatile microorganisms capable of synthesizing a polymer known as Polyhydroxybutyrates (PHBs), which are inclusion bodies that bacteria accumulate as backup materials when they develop under various stressors. PHBs are chosen as substitutes for natural environmental conditions for the manufacturing of biodegradable polymers due to their rapid degradability. This effort aimed to identify putative PHB-synthesizing bacteria and assess PHB productivity with the help of agro-residues as carbon residues. The goal of this investigation was to isolate bacteria that synthesize bioplastic from plastic waste dump soil. Following the collection of soil samples, bacterial strains were isolated using culture-based techniques. Four of the ten isolated bacterial strains were shown to be capable of producing bioplastic. Sudan-black staining was used for bioplastic manufacturing screening. The bacterial strain that has shown to be the hyper-producer of PHB was identified by 16S rDNA sequencing as <i>Bacillus cereus</i> ARD-03. For maximum production of PHB, the optimal pH, temperature, and time course of fermentation were found to be 7.5, 30&#xa0;°C, and 24&#xa0;h respectively. When <i>Bacillus cereus</i> ARD-03 was fed on 4&#xa0;g% of each of the agricultural wastes including sugarcane bagasse, waste paper, and rose petals, weight of PHB produced was calculated as 1.577&#xa0;g/dL, 0.304&#xa0;g/dL, and 13.161&#xa0;g/dL respectively. Fourier Transform Infrared Spectroscopy (FTIR) was used to characterize the isolated polymer that confirmed the production of Polyhydroxybutyrate (PHB). The <i>Bacillus cereus</i> ARD-03 isolated in this study, can be utilized to produce PHB based bioplastic economically from agro-residues that can reduce the cost of and ease the substrates’ disposal issues.</p>

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Process Engineering for Sustainable Bioplastic Production Using Bacillus cereus ARD-03 and Agricultural Biomass

  • 期刊论文

  • 发表日期：

    2025年5月9日

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  • Aliya Riaz,
  • Sana Ahmad,
  • Aisha Bibi,
  • Sania Tanvir,
  • Samina Khan,
  • Ayisha Aman Ullah

摘要

Bioplastics are the polymers synthesized from either microorganisms or they can be manufactured using low cost agricultural biomass. They are gaining considerable attention nowadays due to their biodegradability and provision of utilizing waste material. Bacteria are versatile microorganisms capable of synthesizing a polymer known as Polyhydroxybutyrates (PHBs), which are inclusion bodies that bacteria accumulate as backup materials when they develop under various stressors. PHBs are chosen as substitutes for natural environmental conditions for the manufacturing of biodegradable polymers due to their rapid degradability. This effort aimed to identify putative PHB-synthesizing bacteria and assess PHB productivity with the help of agro-residues as carbon residues. The goal of this investigation was to isolate bacteria that synthesize bioplastic from plastic waste dump soil. Following the collection of soil samples, bacterial strains were isolated using culture-based techniques. Four of the ten isolated bacterial strains were shown to be capable of producing bioplastic. Sudan-black staining was used for bioplastic manufacturing screening. The bacterial strain that has shown to be the hyper-producer of PHB was identified by 16S rDNA sequencing as Bacillus cereus ARD-03. For maximum production of PHB, the optimal pH, temperature, and time course of fermentation were found to be 7.5, 30 °C, and 24 h respectively. When Bacillus cereus ARD-03 was fed on 4 g% of each of the agricultural wastes including sugarcane bagasse, waste paper, and rose petals, weight of PHB produced was calculated as 1.577 g/dL, 0.304 g/dL, and 13.161 g/dL respectively. Fourier Transform Infrared Spectroscopy (FTIR) was used to characterize the isolated polymer that confirmed the production of Polyhydroxybutyrate (PHB). The Bacillus cereus ARD-03 isolated in this study, can be utilized to produce PHB based bioplastic economically from agro-residues that can reduce the cost of and ease the substrates’ disposal issues.