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<p>This study investigated the in vitro acaricidal potential of crude leaf extracts of <i>Datura stramonium</i> prepared using aqueous, ethanolic, and hydro-ethanolic solvents against the cattle tick <i>Rhipicephalus microplus</i>. The objective was to assess their efficacy as potential botanical alternatives for tick control. Bioassays were performed using the Adult Immersion Test (AIT) on fully engorged female ticks at concentrations of 800, 400, 200, 100, and 50&#xa0;mg/ml, and the Larval Packet Test (LPT) on unfed larvae at concentrations ranging from 3200 to 6.25&#xa0;mg/ml. In adult ticks, the ethanolic and hydro-ethanolic extracts induced the highest adult tick mortality at 800&#xa0;mg/ml, with calculated LC<sub>50</sub> values of 388.8 and 347.9&#xa0;mg/ml, respectively. The aqueous extract showed minimal adulticidal activity. In larval assays, the hydro-ethanolic extract resulted in 100% mortality at the highest concentration tested (3200&#xa0;mg/ml), while the aqueous and ethanolic extracts caused 72.0 and 63.0% mortality, respectively. The LC<sub>50</sub> values for larvae were determined as 1456&#xa0;mg/ml (hydro-ethanolic), 1158&#xa0;mg/ml (aqueous), and 642.2&#xa0;mg/ml (ethanolic). Among all the extracts, the hydro-ethanolic preparation exhibited the most consistent and potent acaricidal activity against both developmental stages, based on the mean adult tick mortality in the Adult Immersion Test (AIT) and the mean larval mortality in the Larval Packet Test (LPT). These findings suggest that <i>Datura stramonium</i>, particularly its hydro-ethanolic extract, holds promise as a plant-based acaricidal agent.</p>

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Exploring the acaricidal potential of Datura stramonium leaf extracts against Rhipicephalus microplus

  • 期刊论文

  • 发表日期：

    2026年7月24日

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  • Kale Chandrakant Dinkar,
  • Amit Kumar Jaiswal,
  • Supriya Sachan,
  • Soumen Choudhury,
  • Vivek Agrawal,
  • Amit Singh

摘要

This study investigated the in vitro acaricidal potential of crude leaf extracts of Datura stramonium prepared using aqueous, ethanolic, and hydro-ethanolic solvents against the cattle tick Rhipicephalus microplus. The objective was to assess their efficacy as potential botanical alternatives for tick control. Bioassays were performed using the Adult Immersion Test (AIT) on fully engorged female ticks at concentrations of 800, 400, 200, 100, and 50 mg/ml, and the Larval Packet Test (LPT) on unfed larvae at concentrations ranging from 3200 to 6.25 mg/ml. In adult ticks, the ethanolic and hydro-ethanolic extracts induced the highest adult tick mortality at 800 mg/ml, with calculated LC50 values of 388.8 and 347.9 mg/ml, respectively. The aqueous extract showed minimal adulticidal activity. In larval assays, the hydro-ethanolic extract resulted in 100% mortality at the highest concentration tested (3200 mg/ml), while the aqueous and ethanolic extracts caused 72.0 and 63.0% mortality, respectively. The LC50 values for larvae were determined as 1456 mg/ml (hydro-ethanolic), 1158 mg/ml (aqueous), and 642.2 mg/ml (ethanolic). Among all the extracts, the hydro-ethanolic preparation exhibited the most consistent and potent acaricidal activity against both developmental stages, based on the mean adult tick mortality in the Adult Immersion Test (AIT) and the mean larval mortality in the Larval Packet Test (LPT). These findings suggest that Datura stramonium, particularly its hydro-ethanolic extract, holds promise as a plant-based acaricidal agent.