Scholarlink

<p><i>Naegleria fowleri</i>, a free-living waterborne amoeba, causes fatal primary amoebic meningoencephalitis (PAM) when it enters the brain through the nose. Treatment options are limited due to a lack of FDA-approved drugs and poor blood–brain barrier penetration. Silver nanoparticles exhibit potent antiamoebic properties and can enhance the efficacy of therapeutic agents at specific biological targets. Natural folk remedies have long been utilised in disease treatment, with phytochemicals from various plants demonstrating significant amoebicidal properties. These bioactive compounds offer potential therapeutic benefits against amoebic infections. In this study, malabaricones A, B, and C, extracted from Myristica cinnamomea, were conjugated with silver nanoparticles (AgNO<sub>3</sub>) to determine their effects on PAM infection. These AgNPs coated with malabaricones (AgNPs-Mal-A, B, C) were characterised by using UV–visible spectrophotometry and gave a maximum absorbance at 422, 436, and 457 nm, while high-resolution transmission electron microscopy showed the nanoconjugates were regular and rounded in shape, respectively. These nanoconjugates were evaluated against trophozoites of <i>N. fowleri</i>. All three nanoconjugates showed potent activity; however, based on IC₅₀ values, Mal A–AgNPs demonstrated the highest potency, followed by Mal B–AgNPs and Mal C–AgNPs. While Mal C–AgNPs exhibited stronger effects at higher concentrations, its overall potency appears lower compared to Mal A–AgNPs. The morphological changes were recorded via scanning electron microscopy, while viability was illustrated via light microscopy. All nanoconjugates significantly reduced the host cell cytopathogenicity but showed moderate toxicity at lower concentrations against HaCaT cells. The results indicate that all three tested nanoconjugates show potential as effective alternative therapeutic agents against <i>N. fowleri</i> infections.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Natural compound malabaricone conjugated with silver nanoparticle as an antiamoebic agent against primary Amoebic Meningoencephalitis (PAM) caused by Naegleria fowleri

  • 期刊论文

  • 发表日期：

    2025年12月17日

查看全文
  • Kavitha Rajendran,
  • Devandran Apparasamy,
  • Niwasini Krishna Kumar,
  • Usman Ahmed,
  • Haema Thevanayagam,
  • Muhamad Aqmal Othman,
  • Ayaz Anwar

摘要

Naegleria fowleri, a free-living waterborne amoeba, causes fatal primary amoebic meningoencephalitis (PAM) when it enters the brain through the nose. Treatment options are limited due to a lack of FDA-approved drugs and poor blood–brain barrier penetration. Silver nanoparticles exhibit potent antiamoebic properties and can enhance the efficacy of therapeutic agents at specific biological targets. Natural folk remedies have long been utilised in disease treatment, with phytochemicals from various plants demonstrating significant amoebicidal properties. These bioactive compounds offer potential therapeutic benefits against amoebic infections. In this study, malabaricones A, B, and C, extracted from Myristica cinnamomea, were conjugated with silver nanoparticles (AgNO3) to determine their effects on PAM infection. These AgNPs coated with malabaricones (AgNPs-Mal-A, B, C) were characterised by using UV–visible spectrophotometry and gave a maximum absorbance at 422, 436, and 457 nm, while high-resolution transmission electron microscopy showed the nanoconjugates were regular and rounded in shape, respectively. These nanoconjugates were evaluated against trophozoites of N. fowleri. All three nanoconjugates showed potent activity; however, based on IC₅₀ values, Mal A–AgNPs demonstrated the highest potency, followed by Mal B–AgNPs and Mal C–AgNPs. While Mal C–AgNPs exhibited stronger effects at higher concentrations, its overall potency appears lower compared to Mal A–AgNPs. The morphological changes were recorded via scanning electron microscopy, while viability was illustrated via light microscopy. All nanoconjugates significantly reduced the host cell cytopathogenicity but showed moderate toxicity at lower concentrations against HaCaT cells. The results indicate that all three tested nanoconjugates show potential as effective alternative therapeutic agents against N. fowleri infections.