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<p>This study demonstrates the efficacy of loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays, both targeting the major surface protein 5 (msp5) gene, for the detection of <i>Anaplasma marginale</i> in Indian water buffaloes. The LAMP assay exhibited superior sensitivity, detecting the pathogen in 24 out of 110 samples (21.81%), compared to PCR, which identified 16 out of 110 samples (14.55%), and conventional microscopic examination, which detected only 6 out of 110 samples (5.45%). Relative to PCR, the LAMP assay achieved a diagnostic sensitivity of 93.75% (95% CI 69.77–99.84%) and specificity of 90.43% (95% CI 82.60–95.53%), with a positive predictive value of 62.50% and a negative predictive value of 98.84%. These findings highlight the LAMP assay as a sensitive, specific, and practical diagnostic tool for <i>A</i>. <i>marginale</i> in buffaloes, particularly suitable for field conditions due to its simplicity and visual detection capabilities.</p>

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Rapid visual detection of tick borne Anaplasma marginale in Indian buffaloes using loop mediated isothermal amplification assay targeting major surface protein 5 gene

  • 期刊论文

  • 发表日期：

    2025年8月13日

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  • Niranjan Kumar,
  • Jayesh Babulal Solanki,
  • Dharmeshkumar Chandrakantbhai Patel

摘要

This study demonstrates the efficacy of loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays, both targeting the major surface protein 5 (msp5) gene, for the detection of Anaplasma marginale in Indian water buffaloes. The LAMP assay exhibited superior sensitivity, detecting the pathogen in 24 out of 110 samples (21.81%), compared to PCR, which identified 16 out of 110 samples (14.55%), and conventional microscopic examination, which detected only 6 out of 110 samples (5.45%). Relative to PCR, the LAMP assay achieved a diagnostic sensitivity of 93.75% (95% CI 69.77–99.84%) and specificity of 90.43% (95% CI 82.60–95.53%), with a positive predictive value of 62.50% and a negative predictive value of 98.84%. These findings highlight the LAMP assay as a sensitive, specific, and practical diagnostic tool for A. marginale in buffaloes, particularly suitable for field conditions due to its simplicity and visual detection capabilities.