<p>Present study deals with the syntheses of larnite (Ca₃SiO₄) specimen using citric acid and l-alanine as fuels or reducing agents separately while employing sol–gel combustion technique. After decomposition of the gel followed by calcination, the molecular structure and purity of larnite was evaluated by Powder X-ray diffraction (PXRD) and Fourier transform infrared (FT-IR). Afterwards, nano titania (TiO₂) was embedded with larnite to obtain a set of four larnite/TiO₂ samples of composite in assorted compositions, <i>i.e.,</i> L-AL + TiO₂ (50:50), L-AL + TiO₂ (70:30), L-C + TiO₂ (50:50) and L-C + TiO₂ (70:30). The formation of larnite/TiO₂ composite samples were confirmed by PXRD and FT-IR. FE-SEM and TEM micrographs exhibited surface morphology and EDS demonstrated the elemental composition. The biomineralization study revealed that composites were found completely covered with hydroxyapatite till the ninth day of immersion in simulated body fluid. Further, using the well diffusion technique, four samples of larnite/nano titania composites were tested for their antibacterial properties against four infectious microbial species, including <i>Staphylococcus aureus, E. coli, Klebsiella pneumonia</i> and <i>Bacillus subtilis</i>; and against <i>Candida albicans</i> for their antifungal properties. Out of specimen obtained from both the fuels, 50:50 larnite/TiO₂ composites demonstrated effective antibacterial and antifungal activity against each of the four bacterial infections and one common fungal infection. The IC₅₀ values of 50:50 larnite/nano titania composites were observed as 41.03&#xa0;μg/mL and 40.02&#xa0;μg/mL for l-alanine and citric acid fuels, respectively.</p> Graphical Abstract <p></p>