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<p><i>Campylobacter jejuni</i> (<i>C. jejuni</i>) remains a major global foodborne pathogen, with poultry serving as a primary reservoir. This study evaluates the probiotic potential of <i>Ligilactobacillus salivarius</i> Lbs57 against foodborne pathogen <i>C. jejuni</i>. In vitro, the <i>L. salivarius</i> Lbs57 cell-free supernatant (CFS) exhibited broad-spectrum, thermostable antibacterial activity, with the strongest inhibition against <i>C. jejuni</i>. Characterization revealed the activity was pH and protease-sensitive, and resistant to chemical detergents. Multi-omics analysis, including whole-genome sequencing and peptidomics, identified a two-peptide bacteriocin, salivaricin, as the key antimicrobial agent. In chickens, prophylaxis, continuous, and post-colonization interventions significantly reduced <i>C. jejuni</i> cecal colonization, with achieving a 3-log reduction. Furthermore, <i>L. salivarius</i> Lbs57 modulated the cecal microbiota by increasing diversity and enriching beneficial genera (e.g., <i>Bacteroides</i>, <i>Faecalibacterium</i>) while suppressing pathogens like <i>Clostridium</i> and <i>Streptococcus</i>. Overall, these findings highlight the efficacy of <i>L. salivarius</i> Lbs57 is a promising probiotic candidate for limiting <i>C. jejuni</i> and enhancing gut health in poultry.</p>

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Ligilactobacillus salivarius Lbs57 Exerted Antibacterial Activity and Modulated Cecal Microbiota to Reduce Foodborne Pathogen Campylobacter jejuni

  • 期刊论文

  • 发表日期：

    2026年7月3日

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  • Yifang Cui,
  • Xu Feng,
  • Yuxin Shao,
  • Jiahe Chen,
  • Fangfang Guo,
  • Dongyan Zhang,
  • Fuzhou Xu

摘要

Campylobacter jejuni (C. jejuni) remains a major global foodborne pathogen, with poultry serving as a primary reservoir. This study evaluates the probiotic potential of Ligilactobacillus salivarius Lbs57 against foodborne pathogen C. jejuni. In vitro, the L. salivarius Lbs57 cell-free supernatant (CFS) exhibited broad-spectrum, thermostable antibacterial activity, with the strongest inhibition against C. jejuni. Characterization revealed the activity was pH and protease-sensitive, and resistant to chemical detergents. Multi-omics analysis, including whole-genome sequencing and peptidomics, identified a two-peptide bacteriocin, salivaricin, as the key antimicrobial agent. In chickens, prophylaxis, continuous, and post-colonization interventions significantly reduced C. jejuni cecal colonization, with achieving a 3-log reduction. Furthermore, L. salivarius Lbs57 modulated the cecal microbiota by increasing diversity and enriching beneficial genera (e.g., Bacteroides, Faecalibacterium) while suppressing pathogens like Clostridium and Streptococcus. Overall, these findings highlight the efficacy of L. salivarius Lbs57 is a promising probiotic candidate for limiting C. jejuni and enhancing gut health in poultry.