Scholarlink

Background <p>Astragalus polysaccharides (APS) are mostly degraded in the intestine by polysaccharide-degrading enzymes secreted by gut microbiota, and have potential prebiotic properties. Lactic acid bacteria (LAB) fermentation technology has been used for improvement of functional properties of polysaccharides.</p> Objective <p>Thus, the fermentation characteristics and prebiotic activity of Lactic acid bacteria-Astragalus polysaccharide ferments (LAF) were evaluated in this work.</p> Methods &amp; Results <p>The results revealed that fermentation significantly reduced the total sugar content from 75.2% to 48.0%, partially degraded polysaccharides as evidenced by the emergence of lower molecular weight fractions of 16.7&#xa0;kDa, 2.46&#xa0;kDa, 1.36&#xa0;kDa, and 1.12&#xa0;kDa, and markedly increased organic acid production, with lactic acid content rising approximately 24-fold from 2.26 to 53.46&#xa0;mg·g⁻¹ and acetic acid increasing 6.3-fold from 1.54 to 9.65&#xa0;mg·g⁻¹. In the colon, LAF administration restored the population of goblet cells, enhanced the expression of the tight junction protein Zonula occludens 1 and the mucus layer structural protein Mucin 2, and significantly elevated the concentrations of acetic acid, propionic acid, and n-butyric acid compared to the natural recovery group. Furthermore, LAF restored the abundance and diversity of gut microbiota in mice, leading to a significant increase in beneficial genera including <i>Ruminococcus gauvreauii group</i>, <i>Bacteroides</i>, <i>Robinsoniella</i>, and <i>Phascolarctobacterium</i>, while downregulating detrimental bacteria such as <i>Citrobacter</i> and <i>Parasutterella</i>.</p> Conclusions <p>This study provided fundamental data for exploring the prebiotic effects of LAF in modulating gut microbiota and offered a novel avenue for the utilization of LAB and Traditional Chinese Medicine polysaccharides.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Unlocking Gut Health: Evaluation of Structure and Characteristics of Astragalus Polysaccharides Modified by Lactic Acid Bacteria Fermentation

  • 期刊论文

  • 发表日期：

    2026年3月19日

查看全文
  • Pengshuo Yang,
  • Qing Zhou,
  • Mingyue Jia,
  • Kelin Li,
  • Qingsong Qu,
  • Weishuo Ren,
  • Xinhui Peng,
  • Guoqing Li,
  • Yueyang Xie,
  • Bingxuan Li,
  • Xinyuan Shi

摘要

Background

Astragalus polysaccharides (APS) are mostly degraded in the intestine by polysaccharide-degrading enzymes secreted by gut microbiota, and have potential prebiotic properties. Lactic acid bacteria (LAB) fermentation technology has been used for improvement of functional properties of polysaccharides.

Objective

Thus, the fermentation characteristics and prebiotic activity of Lactic acid bacteria-Astragalus polysaccharide ferments (LAF) were evaluated in this work.

Methods & Results

The results revealed that fermentation significantly reduced the total sugar content from 75.2% to 48.0%, partially degraded polysaccharides as evidenced by the emergence of lower molecular weight fractions of 16.7 kDa, 2.46 kDa, 1.36 kDa, and 1.12 kDa, and markedly increased organic acid production, with lactic acid content rising approximately 24-fold from 2.26 to 53.46 mg·g⁻¹ and acetic acid increasing 6.3-fold from 1.54 to 9.65 mg·g⁻¹. In the colon, LAF administration restored the population of goblet cells, enhanced the expression of the tight junction protein Zonula occludens 1 and the mucus layer structural protein Mucin 2, and significantly elevated the concentrations of acetic acid, propionic acid, and n-butyric acid compared to the natural recovery group. Furthermore, LAF restored the abundance and diversity of gut microbiota in mice, leading to a significant increase in beneficial genera including Ruminococcus gauvreauii group, Bacteroides, Robinsoniella, and Phascolarctobacterium, while downregulating detrimental bacteria such as Citrobacter and Parasutterella.

Conclusions

This study provided fundamental data for exploring the prebiotic effects of LAF in modulating gut microbiota and offered a novel avenue for the utilization of LAB and Traditional Chinese Medicine polysaccharides.